Kilgore Augustus M, Welsh Seth, Cheney Elizabeth E, Chitrakar Alisha, Blain Trevor J, Kedl Benjamin J, Hunter Chris A, Pennock Nathan D, Kedl Ross M
Department of Immunology and Microbiology, School of Medicine, University of Colorado Denver at Anschutz Medical Campus, Denver, CO 80045.
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104.
Immunohorizons. 2018 Jan 1;2(1):1-11. doi: 10.4049/immunohorizons.1700054.
It is well accepted that the innate response is a necessary prerequisite to the formation of the adaptive response. This is true for T cell responses against infections or adjuvanted subunit vaccination. However, specific innate parameters with predictive value for the magnitude of an adjuvant-elicited T cell response have yet to be identified. We previously reported how T cell responses induced by subunit vaccination were dependent on the cytokine IL-27. These findings were unexpected, given that T cell responses to an infection typically increase in the absence of IL-27. Using a novel IL-27p28-eGFP reporter mouse, we now show that the degree to which an adjuvant induces IL-27p28 production from dendritic cells and monocytes directly predicts the magnitude of the T cell response elicited. To our knowledge, these data are the first to identify a concrete innate correlate of vaccine-elicited cellular immunity, and they have significant practical and mechanistic implications for subunit vaccine biology.
人们普遍认为，先天反应是适应性反应形成的必要前提。对于针对感染或佐剂亚单位疫苗接种的T细胞反应而言，确实如此。然而，对于佐剂引发的T细胞反应强度具有预测价值的特定先天参数尚未确定。我们之前报道了亚单位疫苗接种诱导的T细胞反应如何依赖于细胞因子IL-27。鉴于在没有IL-27的情况下，T细胞对感染的反应通常会增强，这些发现出乎意料。现在，我们使用一种新型的IL-27p28-eGFP报告小鼠表明，佐剂诱导树突状细胞和单核细胞产生IL-27p28的程度直接预测了所引发的T细胞反应的强度。据我们所知，这些数据首次确定了疫苗引发的细胞免疫的具体先天关联，并且它们对亚单位疫苗生物学具有重要的实际和机制意义。
