Zhang Jiang-ling, Chen Jie, Wang Xiang-rui, Li Wei-wei, Wang Bei-lei, Zhou Jie
Department of Anesthesiology, The Affiliated Renji Hospital of Shanghai Jiaotong University Medical College, Shanghai 200127, China.
Zhen Ci Yan Jiu. 2010 Jun;35(3):182-7.
To investigate the effects of "Neiguan" (PC 6)-electroacupunture (EA) preconditioning on the myocardium and its mast cells in myocardial ischemia/reperfusion (MI/R) rats.
Eighteen male SD rats were randomly assigned to sham group, model (IR) group and EA group (n=6/ group). MI/R model was established by occlusion of the descending anterior branch of the coronary artery. Blood samples were taken from the femoral vein before MI (T0), EA for 30 min (T1), 30 min after MI (T2), 30 min after MI/R (T3) and 120 min after MI/R (T4) for assaying serum tumor necrosis factor (TNF)-alpha and histamine contents by using ELISA. Serum lactate dehydrogenase (LDH) and creatinkinase isoenzyme (CK-MB) levels were measured at T0, T3 and T4 by using an automatic biochemistry analyzer. The infarct size was detected by Evan's blue and tetrazolium chloride (TTC) staining. Myocardial TNF-alpha and histamine contents were detected by ELISA. The percentage of mast cell degranulation was determined by toluidine blue staining.
Following MI/R, serum LDH and CK-MB levels at phase T3 and T4, serum TNF-alpha and histamine contents at phase T2 and T3, and myocardial mast cell degranulation rate increased significantly, and myocardial TNF-alpha and histamine contents decreased in model group in comparison with pre-MI/R (P < 0.05). Compared with IR model group, serum LDH and CK-MB levels at phase T3 and T4, myocardial TNF-alpha and histamine contents all decreased significantly (P < 0.05), but serum TNF-al infarct size was remarkably smaller in EA group than that in IR model group (P < 0.05).
"Neiguan" (PC 6)-EA preconditioning has a cardioprotective effect on the ischemia-reperfusion myocardium by promoting mast cell degranulation.
探讨“内关”（PC 6）电针预处理对心肌缺血/再灌注（MI/R）大鼠心肌及其肥大细胞的影响。
将18只雄性SD大鼠随机分为假手术组、模型（IR）组和电针组（每组n = 6）。通过结扎冠状动脉前降支建立MI/R模型。于心肌梗死前（T0）、电针30分钟（T1）、心肌梗死后30分钟（T2）、MI/R后30分钟（T3）和MI/R后120分钟（T4）从股静脉采集血样，采用酶联免疫吸附测定法（ELISA）检测血清肿瘤坏死因子（TNF）-α和组胺含量。在T0、T3和T4时使用自动生化分析仪测量血清乳酸脱氢酶（LDH）和肌酸激酶同工酶（CK-MB）水平。通过伊文思蓝和氯化三苯基四氮唑（TTC）染色检测梗死面积。采用ELISA检测心肌TNF-α和组胺含量。通过甲苯胺蓝染色测定肥大细胞脱颗粒百分比。
与MI/R前相比，模型组在T3和T4期血清LDH和CK-MB水平、T2和T3期血清TNF-α和组胺含量以及心肌肥大细胞脱颗粒率显著升高，而心肌TNF-α和组胺含量降低（P < 0.05）。与IR模型组相比，电针组在T3和T4期血清LDH和CK-MB水平、心肌TNF-α和组胺含量均显著降低（P < 0.05），但电针组梗死面积明显小于IR模型组（P < 0.05）。
“内关”（PC 6）电针预处理通过促进肥大细胞脱颗粒对缺血再灌注心肌具有心脏保护作用。
