Suppr超能文献

使用水凝胶从指纹中收集氨基酸和 DNA。

Collection of amino acids and DNA from fingerprints using hydrogels.

机构信息

Forensic Science, Amsterdam University of Applied Sciences, Weesperzijde 190, 1097 DZ Amsterdam, The Netherlands.

出版信息

Analyst. 2018 Feb 12;143(4):900-905. doi: 10.1039/c7an01692a.

Abstract

The amino acid profile obtained from a fingerprint may provide valuable information on its donor. For forensic scientists, recovering evidence relating to the amino acid profile of a suspect can potentially be valuable for identification and exclusion purposes. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS. This method allows for the amino acid profile analysis of fingerprints while allowing for their increased visualisation at a later stage using the standard method of cyanoacrylation. We will demonstrate this method to also be capable of collecting DNA from fingerprints with a 20-60% yield in comparison to using a conventional cotton swab.

摘要

从指纹中获得的氨基酸图谱可能为其来源提供有价值的信息。对于法医学家来说,恢复与嫌疑人氨基酸图谱有关的证据,对于识别和排除目的可能具有潜在价值。本文详细介绍了使用可交联的葡聚糖-甲基丙烯酰胺溶液形成水凝胶,从表面收集氨基酸,然后用 UPLC-MS 进行提取和定量。这种方法允许分析指纹的氨基酸图谱,同时允许在以后使用氰基丙烯酰胺的标准方法来增加指纹的可视化。我们将证明这种方法还能够从指纹中收集 DNA,与使用传统的棉签相比,其产量为 20-60%。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验