Forensic Science, Amsterdam University of Applied Sciences, Weesperzijde 190, 1097 DZ Amsterdam, The Netherlands.
Analyst. 2018 Feb 12;143(4):900-905. doi: 10.1039/c7an01692a.
The amino acid profile obtained from a fingerprint may provide valuable information on its donor. For forensic scientists, recovering evidence relating to the amino acid profile of a suspect can potentially be valuable for identification and exclusion purposes. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS. This method allows for the amino acid profile analysis of fingerprints while allowing for their increased visualisation at a later stage using the standard method of cyanoacrylation. We will demonstrate this method to also be capable of collecting DNA from fingerprints with a 20-60% yield in comparison to using a conventional cotton swab.
从指纹中获得的氨基酸图谱可能为其来源提供有价值的信息。对于法医学家来说，恢复与嫌疑人氨基酸图谱有关的证据，对于识别和排除目的可能具有潜在价值。本文详细介绍了使用可交联的葡聚糖-甲基丙烯酰胺溶液形成水凝胶，从表面收集氨基酸，然后用 UPLC-MS 进行提取和定量。这种方法允许分析指纹的氨基酸图谱，同时允许在以后使用氰基丙烯酰胺的标准方法来增加指纹的可视化。我们将证明这种方法还能够从指纹中收集 DNA，与使用传统的棉签相比，其产量为 20-60%。
