van Helmond Ward, de Puit Marcel
Department of Forensic Science, Amsterdam University of Applied Sciences, Amsterdam, DZ, The Netherlands.
Digital Technology and Biometrics, Netherlands Forensic Institute, Den Haag, GB, The Netherlands.
Methods Mol Biol. 2019;2030:439-450. doi: 10.1007/978-1-4939-9639-1_33.
UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.
超高效液相色谱-质谱联用（UPLC-MS）是一种常用技术，首先用于分离复杂样品，随后对感兴趣的分子进行定量分析。在此，我们描述了使用具有酰胺固定相的UPLC-MS对从指纹中提取的未衍生化氨基酸进行定量分析。使用三重四极杆质谱/质谱（MS/MS）或飞行时间质谱（TOF-MS）检测器作为检测器。该方法具有简单快速的样品前处理过程，便于对大量样品进行分析。
