van Helmond Ward, O'Brien Vincent, de Jong Robin, van Esch Jan, Oldenhof Sander, de Puit Marcel
Department of Forensic Science, Amsterdam University of Applied Sciences, Amsterdam, The Netherlands.
Digital Technology and Biometrics, Netherlands Forensic Institute, The Hague, The Netherlands.
Methods Mol Biol. 2019;2030:429-438. doi: 10.1007/978-1-4939-9639-1_32.
The amino acid profile obtained from a fingerprint may provide valuable information on its donor. Unfortunately, the collection of chemicals from the fingerprint is often destructive to the fingerprint ridge detail. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS. This method allows for the amino acid profile analysis of fingerprints while allowing for their increased visualization at a later stage using the standard method of cyanoacrylate fuming followed by basic-yellow dyeing.
从指纹中获取的氨基酸谱可能会提供有关其提供者的有价值信息。不幸的是，从指纹中收集化学物质通常会破坏指纹纹路细节。在此，我们详细介绍了使用可交联的葡聚糖 - 甲基丙烯酸酯溶液形成水凝胶，该水凝胶能够从表面收集氨基酸，随后通过超高效液相色谱 - 质谱联用仪进行提取和定量分析。这种方法能够对指纹进行氨基酸谱分析，同时还能在后续阶段通过使用标准的氰基丙烯酸酯熏蒸法，然后进行碱性黄染色，来增强指纹的可视化效果。
