人类干细胞表达泛连接蛋白。
Human stem cells express pannexins.
作者信息
Hainz Nadine, Beckmann Anja, Schubert Madline, Haase Alexandra, Martin Ulrich, Tschernig Thomas, Meier Carola
机构信息
Department of Anatomy and Cell Biology, Saarland University, Kirrberger Straße, Building 61, Saar, 66421, Homburg, Germany.
Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplant and Vascular Surgery, Hannover Medical School, 30625, Hannover, Germany.
出版信息
BMC Res Notes. 2018 Jan 22;11(1):54. doi: 10.1186/s13104-018-3125-z.
OBJECTIVE
Pannexins are channel proteins important for the release of calcium and adenosine triphosphate, which are among other functions involved in early development. Here, the expression of pannexins was investigated in induced pluripotent stem cells derived from human cord blood endothelial cells (hCBiPS2), in hematopoietic stem cell-derived induced pluripotent stem cells (HSC_F1285_T-iPS2) and in human embryonic stem cells (HES-3). The expression of pannexin (Panx) 1-3 mRNAs was analyzed in all three undifferentiated stem cell lines. Stem cells then underwent undirected differentiation into embryoid bodies and were analyzed regarding expression of germ layer-specific genes.
RESULTS
Panx1, Panx2, and Panx3 mRNAs were expressed in all undifferentiated stem cell lines investigated. In comparison, Panx1 showed the highest expression among all pannexins. The undirected differentiation resulted in a mixed germ layer genotype in all three stem cell lines. Whereas the expression of Panx1 was not affected by differentiation, the expression of Panx2 was slightly increased in differentiated hCBiPS2 cells, HSC_F1285_T-iPS2 as well as HES3 cells as compared to their undifferentiated counterparts. A slight increase of Panx3 expression was observed in differentiated hCBiPS2 cells only. In conclusion, pluripotent stem cells express all three pannexin genes.
目的
泛连接蛋白是对钙和三磷酸腺苷释放很重要的通道蛋白，其在早期发育的多种功能中发挥作用。在此，研究了泛连接蛋白在源自人脐血内皮细胞的诱导多能干细胞（hCBiPS2）、造血干细胞衍生的诱导多能干细胞（HSC_F1285_T-iPS2）以及人胚胎干细胞（HES-3）中的表达情况。分析了所有三种未分化干细胞系中泛连接蛋白（Panx）1 - 3 mRNA的表达。然后干细胞进行无定向分化形成胚状体，并分析其胚层特异性基因的表达情况。
结果
在所研究的所有未分化干细胞系中均表达Panx1、Panx2和Panx3 mRNA。相比之下，Panx1在所有泛连接蛋白中表达最高。无定向分化导致所有三种干细胞系均出现混合胚层基因型。虽然Panx1的表达不受分化影响，但与未分化的对应细胞相比，分化后的hCBiPS2细胞、HSC_F1285_T-iPS2细胞以及HES3细胞中Panx2的表达略有增加。仅在分化后的hCBiPS2细胞中观察到Panx3表达略有增加。总之，多能干细胞表达所有三种泛连接蛋白基因。
Zotero 插件