Department of Analytical Chemistry, Faculty of Science and Technology, University of the Basque Country UPV/EHU, P.O. Box 644, 48080 Bilbao, Basque Country, Spain.
Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country UPV/EHU, P.O. Box 450, 01080 Vitoria-Gasteiz, Basque Country, Spain.
Sci Total Environ. 2018 Jun 1;626:441-450. doi: 10.1016/j.scitotenv.2018.01.096. Epub 2018 Feb 19.
In this work, a reddish biocolonization composed mainly by Trentepohlia algae affecting a synthetic building material from a modern building from the 90s located in the Bizkaia Science and Technology Park (Zamudio, North of Spain) was characterized and its ability to accumulate metals coming from the surrounding atmosphere was evaluated. To asses if these biofilms can act as bioindicators of the surrounding metal pollution, a fast non-invasive in situ methodology based on the use of hand-held energy dispersive X-ray fluorescence (HH-ED-XRF) was used. In order to corroborate the in situ obtained conclusions, some fragments from the affected material were taken to analyze the metal distribution by means of micro-energy dispersive X-ray fluorescence spectroscopy (μ-ED-XRF) and to confirm the presence of metal particles deposited on it using Scanning Electron Microscopy coupled to an Energy Dispersive Spectrometer (SEM-EDS). In order to confirm if Trentepohlia algae biofilms growing on the surface of building materials could be a fast way to in situ provide information about the surrounding metal pollution, a second Trentepohlia algae biofilm growing on a different kind of material (sandstone) was analyzed from an older historical building, La Galea Fortress (Getxo, North of Spain).
本工作主要对一种由 Trentepohlia 藻类组成的红色生物结皮进行了研究，该藻类影响了位于西班牙北部比斯开科技园（Zamudio）的一座 90 年代现代建筑的合成建筑材料。同时评估了其从周围大气中积累金属的能力。为了评估这些生物膜是否可以作为周围金属污染的生物指示剂，采用了一种基于手持式能量色散 X 射线荧光（HH-ED-XRF）的快速非侵入式原位方法。为了验证原位获得的结论，从受影响的材料中取出一些碎片，通过微能量色散 X 射线荧光光谱（μ-ED-XRF）分析金属分布，并使用扫描电子显微镜结合能量色散光谱仪（SEM-EDS）确认沉积在其上的金属颗粒的存在。为了确认生长在建筑材料表面的 Trentepohlia 藻类生物膜是否可以成为快速提供周围金属污染信息的原位方法，我们分析了来自西班牙北部 Getxo 的古老历史建筑 La Galea 堡垒的另一种不同材料（砂岩）上生长的 Trentepohlia 藻类生物膜。
