Biostatistics, Biomathematics, Pharmacoepidemiology and Infectious Diseases (B2PhI), Université Versailles Saint-Quentin-en-Yvelines, INSERM, Institut Pasteur, Université Paris-Saclay, Montigny-le-Bretonneux, France.
USC EA 3671 Mycoplasmal and chlamydial infections in humans, Universite de Bordeaux, French National Reference Center for Bacterial STIs, Bordeaux University Hospital, Bordeaux, France.
Sex Transm Infect. 2018 Dec;94(8):616-618. doi: 10.1136/sextrans-2017-053346. Epub 2018 Jan 22.
New molecular techniques have allowed describing groups of bacterial communities in the vagina (community state types (CST)) that could play an important role in (CT) infection. Our aim was to describe the distribution of CST in a population of young women in France.
A cross-sectional study was carried out in June 2015 among anonymous young women attending a STI clinic in Bordeaux, France. Participants provided a vaginal sample for CT screening and sociodemographic data. CT was diagnosed using the Aptima-combo 2 transcription-mediated-amplification assay. Vaginal microbiota composition was characterised using 16S rRNA gene amplicon sequencing.
Microbiota composition and CT status were available for 132 women. CST dominated by (CST-I), (CST-III) and a diversity of anaerobes (CST-IV) represented 37.1%, 38.6% and 22.0% of the sample, respectively. Twenty-one out of 132 women were CT positive. Proportions of CT-positive women were higher for samples belonging to CST-III (21.6%) and CST-IV (17.2%) than to CST-I (8.2%).
Five CST were found in 132 young women from a STI clinic in France. These CSTs were not significantly associated with CT but higher proportions of CT-positive women were found in CST-III and CST-IV, consistent with a previous study in the Netherlands. Though our study lacked statistical power and was cross-sectional, it is a necessary first step to understand the structure of the vaginal microbiota in French women with or without infection before performing in-depth longitudinal studies.
新的分子技术允许描述阴道中细菌群落（群落状态类型（CST）），这些群落可能在衣原体（CT）感染中发挥重要作用。我们的目的是描述法国年轻女性群体中 CST 的分布。
2015 年 6 月，在法国波尔多的一家性传播感染诊所，对匿名的年轻女性进行了一项横断面研究。参与者提供阴道样本进行 CT 筛查和社会人口统计学数据。使用 Aptima-combo 2 转录介导扩增检测法诊断 CT。使用 16S rRNA 基因扩增子测序来描述阴道微生物群组成。
微生物群组成和 CT 状态可用于 132 名女性。以 （CST-I）、 （CST-III）和多种厌氧菌（CST-IV）为主的 CST 分别占样本的 37.1%、38.6%和 22.0%。132 名女性中有 21 名 CT 阳性。属于 CST-III（21.6%）和 CST-IV（17.2%）的 CT 阳性女性比例高于 CST-I（8.2%）。
在法国一家性传播感染诊所的 132 名年轻女性中发现了 5 种 CST。这些 CST 与 CT 无显著相关性，但 CT 阳性女性中 CST-III 和 CST-IV 的比例较高，与荷兰的一项先前研究一致。尽管我们的研究缺乏统计学效力且为横断面研究，但它是在进行深入的纵向研究之前，了解法国感染或未感染 CT 的女性阴道微生物群结构的必要的第一步。
