MacKay Scott, Abdelrasoul Gaser N, Tamura Marcus, Lin Donghai, Yan Zhimin, Chen Jie
Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 1H9, Canada.
National Institute for Nanotechnology, National Research Council, Edmonton, AB T6G 2M9, Canada.
Sensors (Basel). 2017 Sep 18;17(9):2141. doi: 10.3390/s17092141.
With the increased practice of preventative healthcare to help reduce costs worldwide, sensor technology improvement is vital to patient care. Point-of-care (POC) diagnostics can reduce time and lower labor in testing, and can effectively avoid transporting costs because of portable designs. Label-free detection allows for greater versatility in the detection of biological molecules. Here, we describe the use of an impedance-based POC biosensor that can detect changes in the surface modification of a micro-fabricated chip using impedance spectroscopy. Gold nanoparticles (GNPs) have been employed to evaluate the sensing ability of our new chip using impedance measurements. Furthermore, we used impedance measurements to monitor surface functionalization progress on the sensor's interdigitated electrodes (IDEs). Electrodes made from aluminum and gold were employed and the results were analyzed to compare the impact of electrode material. GNPs coated with mercaptoundecanoic acid were also used as a model of biomolecules to greatly enhance chemical affinity to the silicon substrate. The portable sensor can be used as an alternative technology to ELISA (enzyme-linked immunosorbent assays) and polymerase chain reaction (PCR)-based techniques. This system has advantages over PCR and ELISA both in the amount of time required for testing and the ease of use of our sensor. With other techniques, larger, expensive equipment must be utilized in a lab environment, and procedures have to be carried out by trained professionals. The simplicity of our sensor system can lead to an automated and portable sensing system.
随着全球范围内为降低成本而增加预防性医疗保健的实践，传感器技术的改进对患者护理至关重要。即时检测（POC）诊断可以减少检测时间并降低劳动力成本，并且由于其便携设计可以有效避免运输成本。无标记检测在生物分子检测方面具有更大的通用性。在此，我们描述了一种基于阻抗的POC生物传感器的使用，该传感器可以使用阻抗光谱检测微制造芯片表面修饰的变化。金纳米颗粒（GNP）已被用于通过阻抗测量来评估我们新芯片的传感能力。此外，我们使用阻抗测量来监测传感器叉指电极（IDE）上的表面功能化进展。采用了由铝和金制成的电极，并对结果进行分析以比较电极材料的影响。涂有巯基十一烷酸的GNP也被用作生物分子模型，以大大增强对硅基板的化学亲和力。这种便携式传感器可以用作酶联免疫吸附测定（ELISA）和基于聚合酶链反应（PCR）技术的替代技术。该系统在检测所需时间和传感器的易用性方面都优于PCR和ELISA。使用其他技术时，必须在实验室环境中使用大型、昂贵的设备，并且程序必须由训练有素的专业人员进行。我们传感器系统的简单性可以导致一个自动化的便携式传感系统。
