在储存超过三十年后，[具体品种名称]发酵提取物的毒性活性仍持续存在。 （注：原文中var.后缺少具体品种信息）
Persistence of Toxic Activity of Fermentation Extracts from var. after More Than Three Decades of Storage.
作者信息
Galán-Wong Luis Jesús, Gamiño-Hernández Rossana, Fernández-Chapa David, García-Díaz Graciela, De La Garza-Ramos Myriam A, Guajardo-Barbosa Claudio, Luna-Olvera Hugo Alberto
机构信息
Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Ciudad Universitaria, 66450 San Nicolás de los Garza, NL, Mexico.
Centro de Investigación/Facultad de Odontología, Universidad Autónoma de Nuevo León, Calle Dr. Eduardo Aguirre Pequeño y Silao s/n, Colonia Mitras Centro, 64460 Monterrey, NL, Mexico.
出版信息
Int J Microbiol. 2017;2017:5402748. doi: 10.1155/2017/5402748. Epub 2017 Nov 20.
This study was carried out to determine the persistence of toxicity of fermentation extracts of var. after more than three decades of storage. For this purpose, a population of was established. The mortality rate of 20 spore-crystal extracts purified using the acetone-lactose coprecipitation method was measured and evaluated by bioassays according to a modified WHO protocol. The extracts with the highest mortality rate were determined in triplicate by their LD and LD. All extracts showed toxicity at the highest tested dose (1000 ppm) and some, such as strains 3260 and 3501, still killed larvae at doses as low as 0.01 ppm. These data are surprising because no study on the activity of toxic proteins after such a long storage time has been reported.
本研究旨在确定[变种名称]发酵提取物在储存三十多年后的毒性持久性。为此，建立了[相关种群名称]。使用丙酮-乳糖共沉淀法纯化的20种芽孢晶体提取物的死亡率通过生物测定法按照修改后的世卫组织方案进行测量和评估。死亡率最高的提取物通过其半数致死剂量（LD）和半数致死浓度（LD）进行三次测定。所有提取物在最高测试剂量（1000 ppm）下均显示出毒性，一些提取物，如菌株3260和3501，在低至0.01 ppm的剂量下仍能杀死幼虫。这些数据令人惊讶，因为此前尚无关于如此长时间储存后[相关毒素名称]有毒蛋白质活性的研究报道。
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