Małopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, 30-387, Kraków, Poland.
Institute of Environmental Sciences, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland.
Mycorrhiza. 2018 Apr;28(3):235-246. doi: 10.1007/s00572-018-0819-y. Epub 2018 Jan 23.
Phytoremediation of polluted sites can be improved by co-inoculation with mycorrhizal and endophytic fungi. In this study, the effects of single- and co-inoculation of Lactuca serriola with an arbuscular mycorrhizal (AM) fungus, Rhizoglomus intraradices, and endophytic fungi, Mucor sp. or Trichoderma asperellum, on plant growth, vitality, toxic metal accumulation, sesquiterpene lactone production and flavonoid concentration in the presence of toxic metals were evaluated. Inoculation with the AM fungus increased biomass yield of the plants grown on non-polluted and polluted substrate. Co-inoculation with the AM fungus and Mucor sp. resulted in increased biomass yield of plants cultivated on the polluted substrate, whereas co-inoculation with T. asperellum and the AM fungus increased plant biomass on the non-polluted substrate. In the presence of Mucor sp., mycorrhizal colonization and arbuscule richness were increased in the non-polluted substrate. Co-inoculation with the AM fungus and Mucor sp. increased Zn concentration in leaves and roots. The concentration of sesquiterpene lactones in plant leaves was decreased by AM fungus inoculation in both substrates. Despite enhanced host plant costs caused by maintaining symbiosis with numerous microorganisms, interaction of wild lettuce with both mycorrhizal and endophytic fungi was more beneficial than that with a single fungus. The study shows the potential of double inoculation in unfavourable environments, including agricultural areas and toxic metal-polluted areas.
植物修复受污染场地可以通过与菌根和内生真菌的共接种来改善。在这项研究中，评估了单独和共接种叶用莴苣与丛枝菌根（AM）真菌、内根真菌（Rhizophagus intraradices）和内生真菌（毛霉或木霉）对植物生长、活力、有毒金属积累、倍半萜内酯产量和黄酮类浓度的影响，这些植物生长在有毒金属存在的非污染和污染基质上。接种 AM 真菌增加了在非污染和污染基质上生长的植物的生物量产量。AM 真菌和毛霉的共接种导致在污染基质上种植的植物的生物量产量增加，而 AM 真菌和木霉的共接种增加了非污染基质上植物的生物量。在非污染基质中，毛霉的存在增加了菌根定植和丛枝的丰富度。AM 真菌和毛霉的共接种增加了叶片和根部的锌浓度。在两种基质中，AM 真菌的接种都降低了植物叶片中倍半萜内酯的浓度。尽管与众多微生物维持共生关系会增加宿主植物的成本，但野生莴苣与菌根和内生真菌的相互作用比与单一真菌的相互作用更有利。该研究表明，在不利环境下（包括农业区和有毒金属污染区）进行双重接种具有潜力。
