Yang Rong, Qin Zefeng, Wang Jingjing, Zhang Xiaoxia, Xu Song, Zhao Wei, Huang Zhiyong
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
College of Resource and Environmental Sciences, China Agricultural University, Beijing 100193, China.
Microorganisms. 2022 May 17;10(5):1042. doi: 10.3390/microorganisms10051042.
sp. are free-living filamentous fungi which are common in agro-ecosystems. However, few studies thus far have examined the interaction between and arbuscular mycorrhizal (AM) fungi in saline soil and their potential for improving plant stress tolerance. Here, single, dual-inoculated ( MF, AM fungal community or sp.), and non-inoculated maize ( L.) were subjected to different salinity levels (0, 75, 150, and 225 mM NaCl) to test the synergistic effects of dual inoculants on maize plants in different salt stress conditions. Plant performance and metabolic profiles were compared to find the molecular mechanisms underlying plant protection against salt stress. The first experiment revealed that dual inoculation of an AM fungal community and MF improved the biomass and K/Na ratio in maize under non-saline conditions, and generally enhanced AM fungal growth in root and soil under all but the 225 mM NaCl conditions. However, MF inoculant did not influence the structure of AM fungal communities in maize roots. In the second experiment, dual inoculation of sp. and MF increased maize plant biomass, K/Na ratio, and AM fungal growth in root and soil significantly at both 0 and 75 mM NaCl conditions. We identified metabolic compounds differentially accumulated in dual-inoculated maize that may underline their enhanced maize plant tolerance to increasing soil salinity. Our data suggested that the combination of sp. and leads to interactions, which may play a potential role in alleviating the stress and improve crop productivity in salt-affected soils.
某属是自由生活的丝状真菌，在农业生态系统中很常见。然而，迄今为止，很少有研究考察其与盐渍土壤中丛枝菌根（AM）真菌之间的相互作用以及它们提高植物抗逆性的潜力。在这里，对单接种、双接种（某属真菌、AM真菌群落或某属菌种）以及未接种的玉米（玉米品种）施加不同盐度水平（0、75、150和225 mM NaCl），以测试双接种剂在不同盐胁迫条件下对玉米植株的协同效应。比较了植物表现和代谢谱，以找出植物抵御盐胁迫的分子机制。第一个实验表明，在非盐渍条件下，双接种AM真菌群落和某属真菌提高了玉米的生物量和钾/钠比，并且在除225 mM NaCl条件外的所有条件下，总体上促进了根和土壤中AM真菌的生长。然而，某属真菌接种剂并未影响玉米根中AM真菌群落的结构。在第二个实验中，在0和75 mM NaCl条件下，双接种某属菌种和某属真菌显著增加了玉米植株的生物量、钾/钠比以及根和土壤中AM真菌的生长。我们鉴定了双接种玉米中差异积累的代谢化合物，这些化合物可能是其增强玉米植株对土壤盐度增加耐受性的基础。我们的数据表明，某属菌种和某属真菌的组合导致了相互作用，这可能在缓解胁迫和提高盐渍土壤中作物生产力方面发挥潜在作用。
