College of Plant Protection, Southwest University, Chongqing, China.
The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Insect Sci. 2019 Dec;26(6):1029-1036. doi: 10.1111/1744-7917.12570. Epub 2018 Feb 28.
Helicoverpa armigera, cotton bollworm, is one of the most disastrous pests worldwide, threatening various food and economic crops. Functional genomic tools may provide efficient approaches for its management. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system, dependent on a single guide RNA (sgRNA), has been used to induce indels for targeted mutagenesis in cotton bollworm. However, genomic deletions may be more desirable to disrupt the function of noncoding genes or regulatory sequences. By injecting two sgRNAs with Cas9 protein targeting different exons, we obtained predictable genomic deletions of several hundred bases. We achieved this type of modification with different combinations of sgRNA pairs, including HaCad and HaABCC2. Our finding indicated that CRISPR/Cas9 can be used as an efficient tool to engineer genomes with chromosomal deletion in H. armigera.
棉铃虫（Helicoverpa armigera）是世界上最具破坏性的害虫之一，威胁着各种粮食和经济作物。功能基因组工具可能为其管理提供有效的方法。基于单个向导 RNA（sgRNA）的成簇规律间隔短回文重复序列（CRISPR）/CRISPR 相关蛋白 9（Cas9）系统已被用于在棉铃虫中诱导靶标突变的插入缺失。然而，基因组缺失可能更有利于破坏非编码基因或调控序列的功能。通过注射靶向不同外显子的 Cas9 蛋白的两个 sgRNA，我们获得了可预测的几百个碱基的基因组缺失。我们使用不同 sgRNA 对组合实现了这种类型的修饰，包括 HaCad 和 HaABCC2。我们的发现表明，CRISPR/Cas9 可用于在棉铃虫中进行染色体缺失的基因组工程。
