Aggour Reham L, Gamil Lina
Oral Medicine and Periodontology Department, Faculty of Dentistry, October 6th University, 6th October City, Giza Governorate, Egypt.
Microbiology Department, Faculty of Pharmacy, October 6th University, 6th October City, Giza Governorate, Egypt.
Pol J Microbiol. 2017 Mar 30;66(1):31-37. doi: 10.5604/17331331.1235227.
Antimicrobial properties of platelet rich plasma (PRP) against various microorganisms have been recently pointed out. PRP could be an alternative to conventional antibiotics in preventing oral and periodontal infections. We examined whether PRP has in vitro antimicrobial properties against Aggregatibacter actinomycetemcomitans, Prophyromonas gingivalis, Staphylococcus aureus and Candida albicans. PRP and platelet-poor plasma (PPP) were obtained from whole blood of 10 healthy volunteers and 10 periodontitis patients. In vitro laboratory susceptibility was carried out using the modified agar diffusion method by measuring the diameters of inhibition zones on agar plates coated with selected microbial strains. Both calcium chloride (CaCl2) activated and non activated samples were tested. Both activated PRP and PPP, of both patients and controls, effectively inhibited the growth of A. actinomycetemcomitans, P. gingivalis and C. albicans. However, a statistically significant difference in favor of PRP was found indicating more susceptibility to PRP than PPP (p < 0.05). Non activated PRP and PPP exhibited negative zones of inhibition against the studied microorganisms. There was no activity against S. aureus. No statistically significant difference was found between the antimicrobial effects of PRP and/or PPP obtained from patients and controls (p > 0.05). We conclude that PRP is a potentially useful substance against oral and periodontal pathogens. Activated PRP was found to be more active than activated PPP and the activation of coagulation is a fundamental step. Additionally, the antimicrobial activity of PRP and/or PPP seems not to be affected by periodontitis.
近期研究指出了富含血小板血浆（PRP）对多种微生物的抗菌特性。在预防口腔和牙周感染方面，PRP可能是传统抗生素的一种替代物。我们检测了PRP对伴放线聚集杆菌、牙龈卟啉单胞菌、金黄色葡萄球菌和白色念珠菌是否具有体外抗菌特性。从10名健康志愿者和10名牙周炎患者的全血中获取PRP和贫血小板血浆（PPP）。采用改良琼脂扩散法，通过测量涂有选定微生物菌株的琼脂平板上抑菌圈的直径来进行体外实验室药敏试验。对氯化钙（CaCl2）激活和未激活的样本均进行了检测。患者和对照组的激活PRP和PPP均能有效抑制伴放线聚集杆菌、牙龈卟啉单胞菌和白色念珠菌的生长。然而，发现有利于PRP的统计学显著差异，表明这些微生物对PRP的敏感性高于PPP（p < 0.05）。未激活的PRP和PPP对所研究的微生物呈现负抑菌圈。对金黄色葡萄球菌无活性。从患者和对照组获得的PRP和/或PPP的抗菌效果之间未发现统计学显著差异（p > 0.05）。我们得出结论，PRP是一种针对口腔和牙周病原体的潜在有用物质。发现激活的PRP比激活的PPP更具活性，凝血激活是一个基本步骤。此外，PRP和/或PPP的抗菌活性似乎不受牙周炎的影响。
