Aboelsaad Engy, Moustafa Sameh, Amine Amira, Deghady Akram, El-Attar Laila
Department of Microbiology, High Institute of Public Health, Alexandria University, El-Horreya Road 165, Alexandria, 21561, Egypt.
Department of Vascular Surgery, Faculty of Medicine, Alexandria University, Chamblion Street, Alexandria, 21521, Egypt.
Sci Rep. 2025 Apr 30;15(1):15145. doi: 10.1038/s41598-025-97418-0.
Diabetes mellitus is a global public health concern, with diabetic foot infections (DFIs) being common clinical complications among affected patients. Bacterial isolates resistant to commonly used antimicrobial drugs are becoming more prevalent in DFIs. Some research suggests that platelet-rich plasma (PRP) may inhibit bacterial growth, making it a promising biological therapy. Therefore, an in vitro experimental study was conducted on 53 multidrug-resistant (MDR) bacterial strains isolated from DFIs. The isolates were methicillin-resistant Staphylococcus aureus (MRSA), MDR Klebsiella pneumoniae, and MDR Pseudomonas aeruginosa. The antibacterial activity of PRP was assessed using Kirby-Bauer disk diffusion method, broth microdilution method, checkerboard synergy testing, and time-kill assay. The time-kill assay demonstrated that PRP's antibacterial efficacy peaked during the second hour of incubation for MRSA and Pseudomonas aeruginosa, but peaked at the first hour for Klebsiella pneumoniae. However, the PPR's efficiency against all isolates decreased after the peak point, with no antibacterial activity observed at the 24th h of incubation. Additionally, biofilm inhibition and eradication assays revealed that PRP has no effect on biofilm formation. As a result, PRP has the ability to inhibit bacterial growth, although this effect is transient and depends on the bacterial strain.
糖尿病是一个全球性的公共卫生问题，糖尿病足感染（DFIs）是糖尿病患者常见的临床并发症。对常用抗菌药物耐药的细菌分离株在糖尿病足感染中越来越普遍。一些研究表明，富血小板血浆（PRP）可能会抑制细菌生长，使其成为一种有前景的生物疗法。因此，对从糖尿病足感染中分离出的53株多重耐药（MDR）细菌菌株进行了一项体外实验研究。这些分离株包括耐甲氧西林金黄色葡萄球菌（MRSA）、多重耐药肺炎克雷伯菌和多重耐药铜绿假单胞菌。采用 Kirby-Bauer 纸片扩散法、肉汤微量稀释法、棋盘协同试验和时间杀菌试验评估了PRP的抗菌活性。时间杀菌试验表明，PRP对MRSA和铜绿假单胞菌的抗菌效果在孵育的第2小时达到峰值，但对肺炎克雷伯菌在第1小时达到峰值。然而，PRP对所有分离株的抗菌效率在峰值点之后下降，在孵育24小时时未观察到抗菌活性。此外，生物膜抑制和清除试验表明，PRP对生物膜形成没有影响。因此，PRP具有抑制细菌生长的能力，尽管这种作用是短暂的，并且取决于细菌菌株。
