Yao Mengyu, Ma Lun, Li Lihua, Zhang Junying, Lim Rebecca, Chen Wei, Zhang Yu
J Biomed Nanotechnol. 2016 Oct;12(10):1835-51. doi: 10.1166/jbn.2016.2322.
Photodynamic therapy (PDT) has attracted ever-growing attention as a promising modality for cancer treatment. However, due to poor tissue penetration by light, photodynamic therapy has rarely been used for deeply situated tumors. This problem can be solved if photosensitizers are activated by microwaves (MW) that are able to penetrate deeply into tissues. Here, for the first time, we report microwave-induced photodynamic therapy and exploit copper cysteamine nanoparticles as a new type of photosensitizer that can be activated by microwaves to produce singlet oxygen for cancer treatment. Both in vitro and in vivo studies on a rat osteosarcoma cell line (UMR 106-01) have shown significant cell destruction using copper cysteamine (Cu-Cy) under microwave activation. The heating effects and the release of copper ions from Cu-Cy upon MW stimulation are the main mechanisms for the generation of reactive oxygen species that are lethal bullets for cancer destruction. The copper cysteamine nanoparticle-based microwave-induced photodynamic therapy opens a new door for treating cancer and other diseases.
光动力疗法（PDT）作为一种很有前景的癌症治疗方法，已经引起了越来越多的关注。然而，由于光对组织的穿透性较差，光动力疗法很少用于深部肿瘤的治疗。如果光敏剂能被可深入穿透组织的微波（MW）激活，这个问题就能得到解决。在此，我们首次报道了微波诱导光动力疗法，并开发了一种新型光敏剂——半胱胺铜纳米颗粒，它可被微波激活以产生单线态氧用于癌症治疗。对大鼠骨肉瘤细胞系（UMR 106 - 01）进行的体外和体内研究均表明，在微波激活下，使用半胱胺铜（Cu - Cy）可导致显著的细胞破坏。微波刺激下Cu - Cy产生的热效应和铜离子释放是产生活性氧的主要机制，而活性氧是破坏癌细胞的致命“子弹”。基于半胱胺铜纳米颗粒的微波诱导光动力疗法为治疗癌症及其他疾病打开了一扇新的大门。
