Department of Oncology, Affiliated Hospital of Jining Medical University, Jining, 272029, Shandong, China.
Department of Oncology, Affiliated Hospital of Jining Medical University, Jining, 272029, Shandong, China.
Biochimie. 2018 Apr;147:98-104. doi: 10.1016/j.biochi.2018.01.003. Epub 2018 Jan 31.
Micro-RNAs have been reported to play crucial roles in a diversity of cellular processes such as cell proliferation, differentiation and development by regulating the expression of specific genes. They have also been shown to play vital roles in several diseases such as cancer. In the present study, we investigated the role of miR-143-3p in breast cancer. Our results showed that the expression of miR-143-3p is significantly downregulated in breast cancer cells. Upregulation of miR-143-3p inhibited the proliferation and migration of breast cancer cells. Conversely, inhibition of miR-143-3p promoted the proliferation of cancer cells. Bioinformatics analysis and other several experiments revealed MAPK7 as the potential target of miR-143-3p. Quantitative RT-PCR showed that the expression of MAPK7 correlated well with the expression of miR-143. Moreover, the inhibition of MAPK 7 in breast cancer cells abrogated the effects of miR-143 indicating that miR-143-3p-exerted effects on breast cancer are mediated by MAPK7. Takentogether, these results provide strong clues about the therapeutic potential of miR-143-3p in the treatment of breast cancer.
微 RNA 已被报道通过调节特定基因的表达在多种细胞过程中发挥关键作用，如细胞增殖、分化和发育。它们还被证明在几种疾病中发挥重要作用，如癌症。在本研究中，我们研究了 miR-143-3p 在乳腺癌中的作用。我们的结果表明，miR-143-3p 的表达在乳腺癌细胞中显著下调。miR-143-3p 的上调抑制了乳腺癌细胞的增殖和迁移。相反，抑制 miR-143-3p 促进了癌细胞的增殖。生物信息学分析和其他几项实验揭示了 MAPK7 是 miR-143-3p 的潜在靶标。定量 RT-PCR 显示 MAPK7 的表达与 miR-143 的表达密切相关。此外，在乳腺癌细胞中抑制 MAPK7 消除了 miR-143 的作用，表明 miR-143-3p 对乳腺癌的作用是通过 MAPK7 介导的。总之，这些结果为 miR-143-3p 在治疗乳腺癌方面的治疗潜力提供了有力线索。
