Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation; Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", Akademgorodok, Krasnoyarsk 660036, Russian Federation.
Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation; Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", Akademgorodok, Krasnoyarsk 660036, Russian Federation.
Int J Biol Macromol. 2018 May;111:1019-1026. doi: 10.1016/j.ijbiomac.2018.01.130. Epub 2018 Jan 31.
The present study investigates physicochemical, mechanical, and biological properties of polyhydroxyalkanoate (PHA) copolymers containing 4-hydroxybutyrate (4HB) synthesized in Cupriavidus eutrophus B10646 culture. In poly(3-hydroxybutyrate/4-hydroxybutyrate) [P(3HB/4HB)] bipolymers, 4HB varied between 10.4 and 75.0 mol%; in poly(3-hydroxybutyrate/3-hydroxyvalerate/4-hydroxybutyrate) terpolymers, 4HB constituted 28.7-55.6 mol%; and in poly(3-hydroxybutyrate/3-hydroxyvalerate/4-hydroxybutyrate/3-hydroxyhexanoate) quaterpolymers, 4HB varied between 9.3 and 13.3 mol%. The degree of crystallinity of P(3HB/4HB) copolymers decreased consistently with an increase in 4HB content, reaching 38%. The incorporation of 3-hydroxyvalerate and 3-hydroxyhexanoate into copolymers enhanced that effect. The effect of 4HB monomer units on temperature properties of copolymers was exhibited as lowering of the melting temperature and crystallization temperature, which improved the processing-related properties of the copolymers. All copolymers containing 4HB showed enhanced elongation at break compared to poly(3-hydroxybutyrate). Polymer films prepared from PHAs with different chemical composition had similar microstructure and porosity and had no toxic effect on mouse fibroblast NIH 3 T3 cells, proving their high biocompatibility.
本研究考察了在 Cupriavidus eutrophus B10646 培养物中合成的含有 4-羟基丁酸 (4HB) 的聚羟基烷酸酯 (PHA) 共聚物的物理化学、机械和生物学性质。在聚(3-羟基丁酸酯/4-羟基丁酸酯) [P(3HB/4HB)] 双聚物中，4HB 的含量在 10.4 至 75.0 mol% 之间；在聚(3-羟基丁酸酯/3-羟基戊酸酯/4-羟基丁酸酯) 三聚物中，4HB 的含量为 28.7-55.6 mol%；在聚(3-羟基丁酸酯/3-羟基戊酸酯/4-羟基丁酸酯/3-羟基己酸酯) 四聚物中，4HB 的含量在 9.3 至 13.3 mol% 之间。P(3HB/4HB) 共聚物的结晶度随着 4HB 含量的增加而持续降低，达到 38%。3-羟基戊酸酯和 3-羟基己酸酯的掺入进一步增强了这种效果。4HB 单体单元对共聚物温度性能的影响表现为降低熔融温度和结晶温度，从而改善了共聚物的加工相关性能。与聚(3-羟基丁酸酯)相比，所有含有 4HB 的共聚物的断裂伸长率都有所提高。具有不同化学组成的 PHAs 制备的聚合物薄膜具有相似的微观结构和孔隙率，对小鼠成纤维细胞 NIH 3T3 无毒，证明其具有很高的生物相容性。
