Bian Lei, Cai Xiao-Ming, Luo Zong-Xiu, Li Zhao-Qun, Xin Zhao-Jun, Chen Zong-Mao
Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Xihu, Hangzhou, China.
J Econ Entomol. 2018 Apr 2;111(2):629-636. doi: 10.1093/jee/tox370.
The tea leafhopper, Empoasca onukii Matsuda, is a serious pest of the tea plant. E. onukii prefers to inhabit vigorously growing tender tea leaves. The host selection of E. onukii adults may be associated with plant volatile compounds (VOCs). We sought to identify potentially attractive VOCs from tea leaves at three different ages and test the behavioral responses of E. onukii adults to synthetic VOC blends in the laboratory and field to aid in developing an E. onukii adult attractant. In darkness, the fresh or mature tea leaves of less than 1-mo old could attract more leafhoppers than the mature branches (MB) that had many older leaves (leaf age >1 mo). Volatile analysis showed that the VOC composition of the fresh leaves was the same as that of the mature leaves, but linalool and indole were not at detectable levels in VOCs from the MB. Moreover, the mass ratio differed for each common volatile in the three types of tea leaves. When under competition with volatiles from the MB, the leafhoppers showed no significant tropism to each single volatile but could be attracted by the synthetic volatile blend imitating the fresh leaves. With the removal of some volatile components, the effective synthetic volatile blend was mixed with (Z)-3-hexen-1-ol, (Z)-3-hexenyl acetate, and linalool at a mass ratio of 0.6:23:12.6. These three volatiles may be the key components for the host selection of E. onukii adults and could be used as an attractant in tea gardens.
假眼小绿叶蝉（Empoasca onukii Matsuda）是茶树的一种严重害虫。假眼小绿叶蝉更喜欢栖息在生长旺盛的嫩茶叶上。假眼小绿叶蝉成虫的寄主选择可能与植物挥发性化合物（VOCs）有关。我们试图从三种不同叶龄的茶叶中鉴定出具有潜在吸引力的VOCs，并在实验室和田间测试假眼小绿叶蝉成虫对合成VOC混合物的行为反应，以帮助开发假眼小绿叶蝉成虫引诱剂。在黑暗中，叶龄小于1个月的新鲜或成熟茶叶比有许多老叶（叶龄>1个月）的成熟枝条（MB）能吸引更多的叶蝉。挥发性成分分析表明，新鲜叶片的VOC组成与成熟叶片相同，但MB的VOC中芳樟醇和吲哚未达到可检测水平。此外，三种茶叶中每种常见挥发性成分的质量比也不同。当与MB的挥发物竞争时，叶蝉对每种单一挥发物没有明显的趋向性，但可以被模仿新鲜叶片的合成挥发物混合物吸引。去除一些挥发性成分后，有效的合成挥发物混合物由（Z）-3-己烯-1-醇、（Z）-3-己烯基乙酸酯和芳樟醇以0.6:23:12.6的质量比混合而成。这三种挥发物可能是假眼小绿叶蝉成虫寄主选择的关键成分，可作为茶园引诱剂使用。
