Xu Xiuxiu, Cai Xiaoming, Bian Lei, Luo Zongxiu, Li Zhaoqun, Chen Zongmao
Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, China.
Shandong Institute of Pomology, Shandong Academy of Agricultural Sciences, China.
J Econ Entomol. 2017 Dec 5;110(6):2357-2363. doi: 10.1093/jee/tox269.
Plant volatiles help herbivores to locate their hosts, and therefore, they could be used to help develop pesticide-free pest management strategies. To develop an attractant for tea leafhopper (Empoasca onukii), we screened nine tea plant volatile compounds for their attractiveness using Y-tube olfactometer assays. Results indicated that tea leafhoppers significantly preferred ocimene, limonene, (Z)-3-hexenol, and (Z)-3-hexenyl acetate over clean air. These compounds were combined in a blend which lost its attractiveness at concentrations below 10-2 g/ml in liquid paraffin. In field tests, the blend was attractive to leafhoppers only in autumn, but not in summer. Analyses of the tea field background odor showed that all four components of the blend were present at much higher concentrations in summer (0.05-0.001 ng/liter) than in autumn (~10- to 25-fold lower). In field Y-tube bioassays, compared with the tea field background odor, the blend was attractive at a concentration of 10-1 g/ml in liquid paraffin, but not at 10-2 g/ml. These results suggest that field background odor can disrupt the attractiveness of an attractant based on plant volatiles to herbivores.
植物挥发物有助于食草动物找到它们的宿主，因此，可以利用这些挥发物来制定无农药的害虫管理策略。为了开发一种茶小绿叶蝉（假眼小绿叶蝉）引诱剂，我们使用Y型嗅觉仪试验筛选了9种茶树挥发性化合物的引诱力。结果表明，与洁净空气相比，茶小绿叶蝉显著更喜欢罗勒烯、柠檬烯、（Z）-3-己烯醇和（Z）-3-己烯基乙酸酯。这些化合物混合后，在液体石蜡中浓度低于10-2克/毫升时失去了引诱力。在田间试验中，该混合物仅在秋季对叶蝉有引诱作用，而在夏季则没有。茶园背景气味分析表明，混合物的所有四种成分在夏季（0.05 - 0.001纳克/升）的浓度远高于秋季（低约10至25倍）。在田间Y型嗅觉生物测定中，与茶园背景气味相比，该混合物在液体石蜡中浓度为10-1克/毫升时有引诱作用，但在10-2克/毫升时没有。这些结果表明，田间背景气味会破坏基于植物挥发物的引诱剂对食草动物的引诱力。
