对来自巴基斯坦巴哈瓦尔布尔的禾本科选定物种的生物潜力评估。

Evaluation of biological potential of selected species of family Poaceae from Bahawalpur, Pakistan.

作者信息

Fatima Iram, Kanwal Sobia, Mahmood Tariq

机构信息

Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.

Department of Zoology, University of Gujrat Sub-Campus Rawalpindi, Gujrat, Pakistan.

出版信息

BMC Complement Altern Med. 2018 Jan 24;18(1):27. doi: 10.1186/s12906-018-2092-1.

BACKGROUND

Oxidative stress as well as bacterial and fungal infections are common source of diseases while plants are source of medication for curative or protective purposes. Hence, aim of study was to compare the pharmacological potential of seven grass species in two different solvents i.e. ethanol and acetone.

METHODS

Preliminary phytochemical tests were done and antioxidant activities were evaluated using ELISA and their IC50 values and AAI (%) were recorded. ANOVA was used for statistical analyses. DNA damage protection assay was done using p1391Z plasmid DNA and DNA bands were analyzed. Antimicrobial activity was done via disc diffusion method and MIC and Activity Index were determined. Cytotoxic activity was carried out using the brine shrimps' assay and LC50 values were calculated using probit analysis program.

RESULTS

Phytochemical studies confirmed the presence of secondary metabolites in most of the plant extracts. Maximum antioxidant potential was revealed in DiAEE, DiAAE (AAI- 54.54% and 43.24%) and DaAEE and DaAAE (AAI- 49.13% and 44.52%). However, PoAEE and PoAAE showed minimum antioxidant potential (AAI- 41.04% and 34.11%). SaSEE, DiAEE and ElIEE showed very little DNA damage protection activity. In antimicrobial assay, DaAEE significantly inhibited the growth of most of the microbial pathogens (nine microbes out of eleven tested microbes) among ethanol extracts while DaAAE and ImCAE showed maximum inhibition (eight microbes out of eleven tested microbes) among acetone plant extracts. However, PoAEE and PoAAE showed least antimicrobial activity. F. oxysporum and A. niger were revealed as the most resistant micro-organisms. ImCEA and ImCAE showed maximum cytotoxic potential (LC50 11.004 ppm and 7.932 ppm) as compared to the other plant extracts.

CONCLUSION

Fodder grasses also contains a substantial phenols and flavonoids contents along with other secondary metabolites and, hence, possess a significant medicinal value. Ethanol extracts showed more therapeutic potential as compared to the acetone extracts. This study provides experimental evidence that the selected species contains such valuable natural compounds which can be used as medicinal drugs in future.

背景

氧化应激以及细菌和真菌感染是常见的疾病来源，而植物是用于治疗或保护目的药物的来源。因此，本研究的目的是比较七种草种在两种不同溶剂（即乙醇和丙酮）中的药理潜力。

方法

进行了初步的植物化学测试，并使用酶联免疫吸附测定法评估了抗氧化活性，记录了它们的半数抑制浓度（IC50）值和抗氧化活性指数（AAI%）。采用方差分析进行统计分析。使用p1391Z质粒DNA进行DNA损伤保护试验，并分析DNA条带。通过纸片扩散法进行抗菌活性测试，并确定最低抑菌浓度（MIC）和活性指数。使用卤虫试验进行细胞毒性活性测试，并使用概率分析程序计算半数致死浓度（LC50）值。

结果

植物化学研究证实大多数植物提取物中存在次生代谢物。在二色补血草乙醇提取物（DiAEE）、二色补血草丙酮提取物（DiAAE）（AAI分别为54.54%和43.24%）以及狗尾草乙醇提取物（DaAEE）和狗尾草丙酮提取物（DaAAE）（AAI分别为49.13%和44.52%）中显示出最大的抗氧化潜力。然而，早熟禾乙醇提取物（PoAEE）和早熟禾丙酮提取物（PoAAE）显示出最小的抗氧化潜力（AAI分别为41.04%和34.11%）。盐地碱蓬乙醇提取物（SaSEE）、二色补血草乙醇提取物和画眉草乙醇提取物（ElIEE）显示出非常小的DNA损伤保护活性。在抗菌试验中，狗尾草乙醇提取物在乙醇提取物中显著抑制了大多数微生物病原体的生长（11种受试微生物中的9种），而狗尾草丙酮提取物和无芒隐子草丙酮提取物（ImCAE）在丙酮植物提取物中显示出最大抑制作用（11种受试微生物中的8种）。然而，早熟禾乙醇提取物和早熟禾丙酮提取物显示出最小的抗菌活性。尖孢镰刀菌和黑曲霉被发现是最具抗性的微生物。与其他植物提取物相比，无芒隐子草乙醇提取物（ImCEA）和无芒隐子草丙酮提取物显示出最大的细胞毒性潜力（LC50分别为11.004 ppm和7.932 ppm）。