Zarrin Majid, Rashidnia Zeinab, Faramarzi Sama, Harooni Lida
Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Open Access Maced J Med Sci. 2017 Nov 22;5(7):848-851. doi: 10.3889/oamjms.2017.203. eCollection 2017 Dec 15.
The main purpose of the present study was to test the β-tubulin and genes for rapid identification of .
Fifty-one strains including environmental, clinical and reference isolates were tested in this research. PCR was carried out based on and partial gene sequences.
A 198 bp DNA fragment was obtained using gene. PCR amplification of the rodA gene resulted in a 313 bp band. The and genes PCR products exhibited a 100% homology with the associated sequences in the GenBank.
In the present study, we used a PCR approach that was able to discriminate from other related species within the section Fumigati.
本研究的主要目的是检测β-微管蛋白和基因以快速鉴定。
本研究检测了51株菌株，包括环境菌株、临床分离株和参考菌株。基于和部分基因序列进行聚合酶链反应（PCR）。
使用基因获得了一个198 bp的DNA片段。rodA基因的PCR扩增产生了一条313 bp的条带。和基因的PCR产物与GenBank中的相关序列具有100%的同源性。
在本研究中，我们采用了一种PCR方法，该方法能够从烟曲霉组内的其他相关物种中鉴别出。
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