Mizuno Hiroki, Kikuta Junichi, Ishii Masaru
Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, and WPI-Immunology Frontier Research Center, Osaka University, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan.
Histochem Cell Biol. 2018 Apr;149(4):417-422. doi: 10.1007/s00418-018-1638-0. Epub 2018 Jan 23.
There are as many as 200 cell types in the body, and highly sophisticated and varied life phenomena are carried out by cell migration to appropriate places at appropriate times following the appropriate interactions. Recent advances in optical imaging technology using multi-photon excitation microscopy have enabled visualization inside intact bone tissues in living animals without thin sectioning. Using such advanced techniques, the dynamic behaviors of living bone cells on intact bone tissue structures can be elucidated. Here, we focus on recent findings using intravital multi-photon imaging of dynamic biological systems, e.g., bone homeostasis. This novel approach has proven beneficial for understanding the mechanisms underlying the spatiotemporal nature of bone remodeling systems and for evaluating the specific modes of actions of novel drugs currently in development, which will contribute to a new chapter in bone and mineral research.
人体中有多达200种细胞类型，通过在适当的相互作用后，细胞在适当的时间迁移到适当的位置，从而实现高度复杂和多样的生命现象。利用多光子激发显微镜的光学成像技术的最新进展，能够在不进行薄切片的情况下对活体动物完整骨组织内部进行可视化观察。使用这些先进技术，可以阐明完整骨组织结构上活骨细胞的动态行为。在这里，我们重点关注使用活体多光子成像对动态生物系统（如骨稳态）的最新研究结果。这种新方法已被证明有助于理解骨重塑系统时空特性的潜在机制，并有助于评估目前正在研发的新型药物的具体作用模式，这将为骨与矿物质研究开启新的篇章。
