Burla Bo, Muralidharan Sneha, Wenk Markus R, Torta Federico
Singapore Lipidomics Incubator (SLING), Life Sciences Institute, National University of Singapore, Singapore, Singapore.
Singapore Lipidomics Incubator (SLING), Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Methods Mol Biol. 2018;1730:135-162. doi: 10.1007/978-1-4939-7592-1_11.
Sphingolipids are the most diverse class of lipids due to the numerous variations in their structural components. This diversity is also reflected in their extremely different functions. Sphingolipids are not only constituents of cell membranes but have also emerged as key signaling molecules involved in a variety of cellular functions, such as cell growth and differentiation, proliferation, and apoptotic cell death. Lipidomic analyses in clinical research have identified pathways and products of sphingolipid metabolism that are altered in several human pathologies. In this article, we describe how to properly design a lipidomic experiment in clinical research, how to handle plasma and serum samples for this purpose, and how to measure sphingolipids using liquid chromatography-mass spectrometry.
由于其结构成分存在众多变化，鞘脂是种类最多样化的一类脂质。这种多样性也反映在它们极其不同的功能上。鞘脂不仅是细胞膜的组成成分，还已成为参与多种细胞功能（如细胞生长与分化、增殖以及凋亡性细胞死亡）的关键信号分子。临床研究中的脂质组学分析已经确定了鞘脂代谢的途径和产物，这些在几种人类疾病中会发生改变。在本文中，我们描述了如何在临床研究中正确设计脂质组学实验，如何为此处理血浆和血清样本，以及如何使用液相色谱 - 质谱联用技术测量鞘脂。
