Singapore Lipidomics Incubator (SLING), Life Sciences Institute, National University of Singapore, Singapore, Singapore.
Precision Medicine Translational Research Programme and Department of Biochemistry, YLL School of Medicine, National University of Singapore, Singapore, Singapore.
Methods Mol Biol. 2025;2855:225-268. doi: 10.1007/978-1-0716-4116-3_15.
Sphingolipids are the most diverse class of lipids due to the numerous variations in their structural components. This diversity is also reflected in their extremely different functions. Sphingolipids are not only constituents of cell membranes but have emerged as key signaling molecules involved in a variety of cellular functions, such as cell growth and differentiation, proliferation and apoptotic cell death. Lipidomic analyses in clinical research have identified pathways and products of sphingolipid metabolism that are altered in several human pathologies. In this article, we describe how to properly design a lipidomic experiment in clinical research, how to handle plasma and serum samples for this purpose, and how to measure sphingolipids using liquid chromatography-mass spectrometry.
鞘脂是脂质中种类最多的一类，这是由于其结构成分的多样性。这种多样性也反映在它们极其不同的功能上。鞘脂不仅是细胞膜的组成部分，而且已经成为涉及多种细胞功能的关键信号分子，如细胞生长和分化、增殖和凋亡细胞死亡。临床研究中的脂质组学分析已经确定了几种人类疾病中代谢的鞘脂途径和产物发生了改变。在本文中，我们描述了如何在临床研究中正确设计脂质组学实验，如何为此目的处理血浆和血清样本，以及如何使用液相色谱-质谱法测量鞘脂。
