用于人体血液代谢组分析的化学同位素标记液相色谱-质谱联用技术

Chemical Isotope Labeling LC-MS for Human Blood Metabolome Analysis.

作者信息

Han Wei, Li Liang

机构信息

Department of Chemistry, University of Alberta, Edmonton, AB, Canada.

出版信息

Methods Mol Biol. 2018;1730:213-225. doi: 10.1007/978-1-4939-7592-1_14.

DOI:10.1007/978-1-4939-7592-1_14

PMID:29363075

Abstract

Blood is a widely used biofluid in discovery metabolomic research to search for clinical metabolite biomarkers of diseases. Analyzing the entire human blood metabolome is a major analytical challenge, as blood, after being processed into serum or plasma, contains thousands of metabolites with diverse chemical and physical properties as well as a wide range of concentrations. We describe an enabling method based on high-performance chemical isotope labeling (CIL) liquid chromatography-mass spectrometry (LC-MS) for in-depth quantification of the metabolomic differences in comparative blood samples with high accuracy and precision.

摘要

血液是发现代谢组学研究中广泛使用的生物流体，用于寻找疾病的临床代谢物生物标志物。分析整个人类血液代谢组是一项重大的分析挑战，因为血液在被处理成血清或血浆后，含有数千种具有不同化学和物理性质以及广泛浓度范围的代谢物。我们描述了一种基于高性能化学同位素标记（CIL）液相色谱-质谱（LC-MS）的方法，用于高精度和高准确度地深入定量比较血液样本中的代谢组差异。