Department of Chemistry, University of Alberta, Edmonton, AB, Canada.
Adv Exp Med Biol. 2021;1280:1-18. doi: 10.1007/978-3-030-51652-9_1.
Due to the great diversity of chemical and physical properties of metabolites as well as a wide range of concentrations of metabolites present in metabolomic samples, performing comprehensive and quantitative metabolome analysis is a major analytical challenge. Conventional approach of combining various techniques and methods with each detecting a fraction of the metabolome can lead to the increase in overall metabolomic coverage. However, this approach requires extensive investment in equipment and analytical expertise with still relatively low coverage and low sample throughput. Chemical isotope labeling (CIL) liquid chromatography mass spectrometry (LC-MS) offers an alternative means of increasing metabolomic coverage while maintaining high quantification precision and accuracy. This chapter describes the CIL LC-MS method and its key features for metabolomic analysis.
由于代谢物的化学和物理性质具有极大的多样性，以及代谢组学样本中存在广泛浓度范围的代谢物，因此进行全面和定量的代谢组学分析是一个主要的分析挑战。将各种技术和方法结合使用的常规方法，每种方法检测代谢组的一部分，可增加整体代谢组覆盖范围。然而，这种方法需要在设备和分析专业知识方面进行大量投资，而且覆盖率仍然相对较低，样品通量也较低。化学同位素标记（CIL）液相色谱-质谱联用（LC-MS）为在保持高定量精密度和准确度的同时增加代谢组覆盖范围提供了另一种手段。本章描述了 CIL LC-MS 方法及其用于代谢组学分析的关键特征。
