Hoving Lisa R, Heijink Marieke, van Harmelen Vanessa, van Dijk Ko Willems, Giera Martin
Department of Human Genetics and Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
Center for Proteomics and Metabolomics, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
Methods Mol Biol. 2018;1730:257-265. doi: 10.1007/978-1-4939-7592-1_18.
Our body contains a wide variety of fatty acids that differ in chain length, the degree of unsaturation, and location of the double bonds. As the various fatty acids play distinct roles in health and disease, methods that can specifically determine the fatty acid profile are needed for fundamental and clinical studies. Here we describe a method for the separation and quantification of fatty acids ranging from 8 to 24 carbon chain lengths in blood samples using gas chromatography-mass spectrometry following derivatization using pentafluorobenzyl bromide. This method quantitatively monitors fatty acid composition in a manner that satisfies the requirements for comprehensiveness, sensitivity, and accuracy.
我们的身体含有多种脂肪酸，它们在链长、不饱和度以及双键位置上存在差异。由于各种脂肪酸在健康和疾病中发挥着不同的作用，基础研究和临床研究需要能够特异性测定脂肪酸谱的方法。在此，我们描述了一种使用气相色谱 - 质谱联用仪，在经五氟苄基溴衍生化后，分离和定量血液样本中碳链长度从8到24的脂肪酸的方法。该方法以满足全面性、灵敏度和准确性要求的方式定量监测脂肪酸组成。
