Kim Hye Kyong, Kostidis Sarantos, Choi Young Hae
Natural Product Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands.
Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
Methods Mol Biol. 2018;1730:317-328. doi: 10.1007/978-1-4939-7592-1_24.
Fecal analysis can generate data that is relevant for the exploration of gut microbiota and their relationship with the host. Nuclear magnetic resonance (NMR) spectroscopy is an excellent tool for the profiling of fecal extracts as it enables the simultaneous detection of various metabolites from a broad range of chemical classes including, among others, short-chain fatty acids, organic acids, amino acids, bile acids, carbohydrates, amines, and alcohols. Compounds present at low μM concentrations can be detected and quantified with a single measurement. Moreover, NMR-based profiling requires a relatively simple sample preparation. Here we describe the three main steps of the general workflow for the NMR-based profiling of feces: sample preparation, NMR data acquisition, and data analysis.
粪便分析能够生成与肠道微生物群及其与宿主关系探索相关的数据。核磁共振（NMR）光谱法是粪便提取物分析的一种出色工具，因为它能够同时检测来自广泛化学类别的各种代谢物，包括短链脂肪酸、有机酸、氨基酸、胆汁酸、碳水化合物、胺类和醇类等。低至微摩尔浓度的化合物可通过单次测量进行检测和定量。此外，基于NMR的分析需要相对简单的样品制备。在此，我们描述基于NMR的粪便分析通用工作流程的三个主要步骤：样品制备、NMR数据采集和数据分析。
