Gruenke L D, Craig J C, Klein F D, Nguyen T L, Hitzemann B A, Holaday J W, Loh H H, Braff L, Fischer A, Glick I D
Biomed Mass Spectrom. 1985 Dec;12(12):707-13. doi: 10.1002/bms.1200121207.
A method for the quantitative determination of chlorpromazine and five of its major metabolites in a single sample of biological fluid in the ng/ml range has been developed utilizing gas chromatography/mass spectrometry with selected ion recording. The assay is highly specific and quantification is accomplished by an inverse stable isotope dilution technique, using deuterium-labeled variants of the compounds as internal standards. In this way the concentrations of chlorpromazine and five of its major metabolites (the sulfoxide, the N-oxide, the monodemethylated, the didemethylated, and the 7-hydroxylated compounds) can be determined in biological fluids. Levels in humans have been measured both in plasma and in red blood cells and are compared to those found in related in vitro studies.
已开发出一种利用气相色谱/质谱联用选择离子记录技术，在纳克/毫升范围内对生物流体单一样本中的氯丙嗪及其五种主要代谢物进行定量测定的方法。该测定法具有高度特异性，定量通过反相稳定同位素稀释技术完成，使用化合物的氘标记变体作为内标。通过这种方式，可以测定生物流体中氯丙嗪及其五种主要代谢物（亚砜、N-氧化物、单去甲基化、双去甲基化和7-羟基化化合物）的浓度。已测量了人体血浆和红细胞中的水平，并与相关体外研究中的水平进行了比较。
