Gruenke L D, Craig J C, Petrakis N L, Lyon M B
Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco 94143.
Biomed Environ Mass Spectrom. 1987 Jul;14(7):335-8. doi: 10.1002/bms.1200140707.
A method has been developed for the quantitative determination of cholesterol and three of its major oxidative metabolites (the 5 alpha,6 alpha-epoxide, the 3 beta,5 alpha,6 beta-triol, and the 5 beta,6 beta-epoxide) in a single sample of human breast fluid (2-50 microliters), using gas chromatography/mass spectrometry with selected ion monitoring. High specificity and reliable quantification is achieved by the use of the inverse stable isotope dilution method, employing deuterium-labeled variants of the compounds as internal standards.
已开发出一种方法，用于在2 - 50微升的单份人乳样本中定量测定胆固醇及其三种主要氧化代谢物（5α,6α - 环氧化物、3β,5α,6β - 三醇和5β,6β - 环氧化物），该方法采用气相色谱/质谱联用的选择离子监测技术。通过使用逆稳定同位素稀释法，以化合物的氘标记变体作为内标，实现了高特异性和可靠的定量。
