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钙调蛋白刺激脑腺苷酸环化酶所需的可分离蛋白质亚基的证据。

Evidence for a dissociable protein subunit required for calmodulin stimulation of brain adenylate cyclase.

作者信息

Toscano W A, Westcott K R, LaPorte D C, Storm D R

出版信息

Proc Natl Acad Sci U S A. 1979 Nov;76(11):5582-6. doi: 10.1073/pnas.76.11.5582.

DOI:10.1073/pnas.76.11.5582
PMID:293663
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC411693/
Abstract

An adenylate cyclase [ATP pyrophosphatelyase (cyclizing), EC 4.6.1.1] preparation that is not stimulated by NaF,5'-guanylyl imidodiphosphate, or Ca2+.calmodulin has been isolated from bovine cerebral cortex by Affi-Gel Blue chromatography and calmodulin-Sepharose chromatography. Sensitivity to these effectors was restored by incubation of the adenylate cyclase preparation with detergent-solubilized protein from bovine cerebral cortex. Reconstitution of of Ca2+.calmodulin activation required the presence of 5'-guanylyl imidodiphosphate. The factor required for restoration of Ca2+.calmodulin stimulation was sensitive to heat, trypsin digestion, and N-ethylmaleimide. These observations suggest that this adenylate cyclase activity requires the presence of one or more guanyl nucleotide binding subunits for calmodulin sensitivity.

摘要

一种不受氟化钠、5'-鸟苷酰亚氨基二磷酸或钙离子·钙调蛋白刺激的腺苷酸环化酶[ATP焦磷酸裂解酶（环化），EC 4.6.1.1]制剂，已通过Affi-Gel Blue层析和钙调蛋白-琼脂糖层析从牛大脑皮层中分离出来。通过将腺苷酸环化酶制剂与来自牛大脑皮层的去污剂溶解蛋白一起孵育，恢复了对这些效应物的敏感性。钙离子·钙调蛋白激活的重建需要5'-鸟苷酰亚氨基二磷酸的存在。恢复钙离子·钙调蛋白刺激所需的因子对热、胰蛋白酶消化和N-乙基马来酰亚胺敏感。这些观察结果表明，这种腺苷酸环化酶活性需要存在一个或多个鸟苷酸结合亚基才能对钙调蛋白敏感。

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Evidence for a dissociable protein subunit required for calmodulin stimulation of brain adenylate cyclase.钙调蛋白刺激脑腺苷酸环化酶所需的可分离蛋白质亚基的证据。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5582-6. doi: 10.1073/pnas.76.11.5582.
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引用本文的文献

1
GTP is not required for calmodulin stimulation of bovine brain adenylate cyclase.钙调蛋白刺激牛脑腺苷酸环化酶并不需要鸟苷三磷酸（GTP）。
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1462-5. doi: 10.1073/pnas.79.5.1462.
2
Properties of the separated catalytic and regulatory units of brain adenylate cyclase.脑腺苷酸环化酶分离出的催化单元和调节单元的特性
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6344-8. doi: 10.1073/pnas.77.11.6344.
3
Differential effects of Ca2+-calmodulin on adenylate cyclase activity cyclase activity in mouse and rat pancreatic islets.钙离子-钙调蛋白对小鼠和大鼠胰岛中腺苷酸环化酶活性的不同影响。
Biochem J. 1982 Jul 15;206(1):97-102. doi: 10.1042/bj2060097.
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Mechanism of action of choleragen and E. coli heat-labile enterotoxin: activation of adenylate cyclase by ADP-ribosylation.霍乱毒素和大肠杆菌不耐热肠毒素的作用机制：通过ADP-核糖基化激活腺苷酸环化酶。
Mol Cell Biochem. 1981 Jul 7;37(2):75-90. doi: 10.1007/BF02354931.
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Ca2+/calmodulin sensitivity may be common to all forms of neural adenylate cyclase.钙离子/钙调蛋白敏感性可能是所有形式的神经腺苷酸环化酶所共有的。
Proc Natl Acad Sci U S A. 1989 Dec;86(23):9564-8. doi: 10.1073/pnas.86.23.9564.

本文引用的文献

1
Preparation of [alpha-32P]nucleoside and deoxynucleoside 5'-triphosphates from 32Pi and protected and unprotected nucleosides.由³²Pi以及受保护和未受保护的核苷制备[α-³²P]核苷和脱氧核苷5'-三磷酸。
Biochim Biophys Acta. 1969 Oct 22;190(2):548-50. doi: 10.1016/0005-2787(69)90105-1.
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A highly sensitive adenylate cyclase assay.一种高灵敏度的腺苷酸环化酶检测方法。
Anal Biochem. 1974 Apr;58(2):541-8. doi: 10.1016/0003-2697(74)90222-x.
3
Stimulatory and inhibitory effects of guanyl nucleotides on fat cell adenylate cyclase.鸟苷酸对脂肪细胞腺苷酸环化酶的刺激和抑制作用。
J Biol Chem. 1973 Sep 10;248(17):6239-45.
4
Cyclic 3',5'-nucleotide phosphodiesterase. Evidence for and properties of a protein activator.环3',5'-核苷酸磷酸二酯酶。一种蛋白质激活剂的证据及特性
J Biol Chem. 1971 May 10;246(9):2859-69.
5
Activation of pigeon erythrocyte membrane adenylate cyclase by guanylnucleotide analogues and separation of a nucleotide binding protein.鸟苷酸类似物对鸽红细胞膜腺苷酸环化酶的激活作用及一种核苷酸结合蛋白的分离
J Biol Chem. 1975 Feb 10;250(3):867-76.
6
Coupling of catecholamine receptor from one cell with adenylate cyclase from another cell by cell fusion.通过细胞融合将一个细胞的儿茶酚胺受体与另一个细胞的腺苷酸环化酶偶联。
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4410-4. doi: 10.1073/pnas.73.12.4410.
7
Regulation of adenylate cyclase from glial tumor cells by calcium and a calcium-binding protein.钙及一种钙结合蛋白对胶质细胞瘤细胞中腺苷酸环化酶的调节作用
J Biol Chem. 1976 Aug 10;251(15):4744-50.
8
GTP-binding proteins in membranes and the control of adenylate cyclase activity.膜中的GTP结合蛋白与腺苷酸环化酶活性的调控
J Biol Chem. 1977 Oct 25;252(20):7224-34.
9
Resolution of beta-adrenergic receptor binding and adenylate cyclase activity by gel exclusion chromatography.通过凝胶排阻色谱法解析β-肾上腺素能受体结合与腺苷酸环化酶活性
J Biol Chem. 1977 Jan 25;252(2):799-802.
10
Ca++-dependent formation of brain adenylate cyclase-protein activator complex.脑腺苷酸环化酶 - 蛋白激活剂复合物的钙离子依赖性形成
Biochem Biophys Res Commun. 1976 Jan 26;68(2):616-25. doi: 10.1016/0006-291x(76)91190-6.

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