Bissen S T, Ueda T
Neurochem Res. 1986 Mar;11(3):453-63. doi: 10.1007/BF00965018.
The subcellular distribution of Ca2+/calmodulin-stimulated adenylate cyclase activity was studied in comparison with that of guanine nucleotide-stimulated cyclase activity. The distributions of these activities were similar among the crude fractions but differed among the purified subsynaptosomal fractions. The specific activity of Ca2+/calmodulin-stimulated cyclase was highest in a light synaptic membrane fraction, which has few, if any, postsynaptic densities, whereas that of guanine nucleotide-stimulated cyclase was highest in a heavier synaptic membrane fraction rich in postsynaptic densities. These results suggest that the Ca2+/calmodulin-stimulated cyclase has, at least in part, a different cellular or subcellular location than the guanine nucleotide-stimulated cyclase.
研究了钙/钙调蛋白刺激的腺苷酸环化酶活性与鸟嘌呤核苷酸刺激的环化酶活性的亚细胞分布，并进行了比较。这些活性在粗分级分中的分布相似，但在纯化的突触小体下分级分中有所不同。钙/钙调蛋白刺激的环化酶的比活性在轻突触膜分级分中最高，该分级分中几乎没有（如果有的话）突触后致密物，而鸟嘌呤核苷酸刺激的环化酶的比活性在富含突触后致密物的较重突触膜分级分中最高。这些结果表明，钙/钙调蛋白刺激的环化酶至少部分地与鸟嘌呤核苷酸刺激的环化酶具有不同的细胞或亚细胞定位。
