Robert Koch Institute, National Reference Centre for Salmonella and Other Bacterial Enteric Pathogens and Unit of Enteropathogenic Bacteria and Legionella, Wernigerode, Germany.
Robert Koch Institute, National Reference Centre for Salmonella and Other Bacterial Enteric Pathogens and Unit of Enteropathogenic Bacteria and Legionella, Wernigerode, Germany.
Food Microbiol. 2018 May;71:46-54. doi: 10.1016/j.fm.2017.08.017. Epub 2017 Aug 25.
In Germany salmonellosis still represents the 2nd most common bacterial foodborne disease. The majority of infections are caused by Salmonella (S.) Typhimurium and S. Enteritidis followed by a variety of other broad host-range serovars. Salmonella Derby is one of the five top-ranked serovars isolated from humans and it represents one of the most prevalent serovars in pigs, thus bearing the potential risk for transmission to humans upon consumption of pig meat and products thereof. From November 2013 to January 2014 S. Derby caused a large outbreak that affected 145 primarily elderly people. Epidemiological investigations identified raw pork sausage as the probable source of infection, which was confirmed by microbiological evidence. During the outbreak isolates from patients, food specimen and asymptomatic carriers were investigated by conventional typing methods. However, the quantity and quality of available microbiological and epidemiological data made this outbreak highly suitable for retrospective investigation by Whole Genome Sequencing (WGS) and subsequent evaluation of different bioinformatics approaches for cluster definition. Overall the WGS-based methods confirmed the results of the conventional typing but were of significant higher discriminatory power. That was particularly beneficial for strains with incomplete epidemiological data. For our data set both, single nucleotide polymorphism (SNP)- and core genome multilocus sequence typing (cgMLST)-based methods proved to be appropriate tools for cluster definition.
在德国，沙门氏菌病仍然是第二常见的细菌性食源性疾病。大多数感染是由肠炎沙门氏菌（S.）和肠炎沙门氏菌引起的，其次是各种其他广泛宿主范围的血清型。德尔卑沙门氏菌是从人类分离的五个排名最高的血清型之一，它是猪中最常见的血清型之一，因此在食用猪肉及其制品时，有可能将其传播给人类。从 2013 年 11 月到 2014 年 1 月，德尔卑沙门氏菌引发了一次大规模疫情，主要影响了 145 名老年人。流行病学调查将生猪肉香肠确定为可能的感染源，这一结论得到了微生物学证据的证实。在疫情期间，通过常规分型方法对来自患者、食品样本和无症状携带者的分离株进行了调查。然而，现有的微生物学和流行病学数据的数量和质量使得这次疫情非常适合采用全基因组测序（WGS）进行回顾性调查，并随后评估不同的生物信息学方法来定义聚类。总体而言，基于 WGS 的方法证实了传统分型的结果，但具有更高的区分能力。对于那些具有不完全流行病学数据的菌株，这尤其有益。对于我们的数据集，单核苷酸多态性（SNP）和核心基因组多位点序列分型（cgMLST）两种方法都被证明是定义聚类的合适工具。
