A retrospective and regional approach assessing the genomic diversity of Dublin.

From a historically rare serotype, subsp. Dublin slowly became one of the most prevalent in cattle and raw milk cheese in some regions of France. We present a retrospective genomic analysis of 480 . Dublin isolates to address the context, evolutionary dynamics, local diversity and the genesis processes of regional . Dublin outbreaks events between 2015 and 2017. Samples were clustered and assessed for correlation against metadata including isolation date, isolation matrices, geographical origin and epidemiological hypotheses. Significant findings can be drawn from this work. We found that the geographical distance was a major factor explaining genetic groups in the early stages of the cheese production processes (animals, farms) while down-the-line transformation steps were more likely to host genomic diversity. This supports the hypothesis of a generalised local persistence of strains from animal to finished products, with occasional migration. We also observed that the bacterial surveillance is representative of diversity, while targeted investigations without genomics evidence often included unrelated isolates. Combining both approaches in phylogeography methods allows a better representation of the dynamics, of outbreaks.