Department of Chemical Engineering, A.C. Tech Campus, Anna University, Chennai, 600 025, Tamil Nadu, India.
Microb Pathog. 2018 Mar;116:215-220. doi: 10.1016/j.micpath.2018.01.027. Epub 2018 Jan 31.
In recent years, biosynthesis of nanoparticles has received considerable attention due to the growing need to develop clean and nontoxic chemicals, low-cost approaches, eco - friendly solvents and renewable materials. In the current study, the biosynthesis of TiO nanoparticles (TiONPs) was attained by a chemical and biosynthesized method by using the aqueous leaf extract of Trigonella foenum-graecum (TF-TiONP). TiO NPs were characterized by FTIR, UV, XRD, HR-TEM and HR-SEM methods. The X-ray diffraction displayed the existence of TF-TiONPs which is confirmed by the incidence of peaks at 25.28 corresponds to 101 anatase form. HR-SEM perceptions revealed that synthesized TiONPs were spherical in shape and the size of individual nanoparticles as well as a few aggregates was found to be 20-90 nm. The antimicrobial activities of biosynthesized nanoparticles (TF-TiONPs) were examined using Kirby-Bauer method. The TF-TiO nanoparticles showed significant antimicrobial activity against all the tested microorganisms.
近年来，由于开发清洁、无毒的化学品、低成本方法、环境友好型溶剂和可再生材料的需求不断增长，纳米粒子的生物合成受到了相当大的关注。在本研究中，通过使用 Trigonella foenum-graecum（TF）的水提叶提取物，采用化学和生物合成方法获得了 TiO 纳米粒子（TiONPs）的生物合成。TiONPs 通过傅里叶变换红外光谱（FTIR）、紫外线（UV）、X 射线衍射（XRD）、高分辨率透射电子显微镜（HR-TEM）和高分辨率扫描电子显微镜（HR-SEM）方法进行了表征。X 射线衍射显示了 TF-TiONPs 的存在，这通过在 25.28 处出现对应于锐钛矿形式的 101 峰得到了证实。高分辨率扫描电子显微镜观察表明，合成的 TiONPs 呈球形，单个纳米粒子的大小以及一些聚集体的大小发现为 20-90nm。使用 Kirby-Bauer 方法检查了生物合成纳米粒子（TF-TiONPs）的抗菌活性。TF-TiO 纳米粒子对所有测试的微生物均显示出显著的抗菌活性。
