Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan 250100, China.
Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan 250100, China.
Water Res. 2018 Apr 15;133:79-86. doi: 10.1016/j.watres.2018.01.020. Epub 2018 Jan 9.
Pharmaceutical wastewaters containing antibiotics and high salinity can damage traditional biological treatment and result in the proliferation of antibiotic resistance genes (ARGs). Bioelectrochemical system (BES) is a promising approach for treating pharmaceutical wastewater. However, the fate of ARGs in BES and their correlations with microbial communities and horizontal genes transfer are unknown. In this study, we investigated the response of ARGs to bio-electrochemical treatment of chloramphenicol wastewater and their potential hosts under different salinities. Three ARGs encoding efflux pump (cmlA, floR and tetC), one class 1 integron integrase encoding gene (intI1), and sul1 gene (associate with intI1) were analyzed. Correlation analysis between microbial community and ARGs revealed that the abundances of potential hosts of ARGs were strongly affected by salinity, which further determined the alteration in ARGs abundances under different salinities. There were no significant correlations between ARGs and intI1, indicating that horizontal gene transfer was not related to the important changes in ARGs. Moreover, the chloramphenicol removal efficiency was enhanced under a moderate salinity, attributed to the altered microbial community driven by salinity. Therefore, microbial community shift is the major factor for the changes of ARGs and chloramphenicol removal efficiency in BES under different salinities. This study provides new insights on the mechanisms underlying the alteration of ARGs in BES treating high-salinity pharmaceutical wastewater.
含抗生素和高盐度的制药废水会破坏传统的生物处理，并导致抗生素耐药基因（ARGs）的增殖。生物电化学系统（BES）是处理制药废水的一种很有前途的方法。然而，BES 中 ARGs 的命运及其与微生物群落和水平基因转移的相关性尚不清楚。在本研究中，我们研究了不同盐度下生物电化学处理氯霉素废水时 ARGs 对其的响应及其潜在宿主。分析了三个编码外排泵（cmlA、floR 和 tetC）的 ARG、一个 1 类整合子整合酶编码基因（intI1）和与 intI1 相关的 sul1 基因。微生物群落与 ARGs 的相关性分析表明，ARGs 的潜在宿主丰度受盐度强烈影响，这进一步决定了不同盐度下 ARGs 丰度的变化。ARGs 与 intI1 之间没有显著相关性，表明水平基因转移与 ARGs 的重要变化无关。此外，在中等盐度下，氯霉素的去除效率得到了提高，这归因于盐度驱动的微生物群落的改变。因此，在不同盐度下，BES 中微生物群落的变化是 ARGs 和氯霉素去除效率变化的主要因素。本研究为 BES 处理高盐度制药废水时 ARGs 变化的机制提供了新的见解。
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