Vasantharaj S, Sripriya N, Shanmugavel M, Manikandan E, Gnanamani A, Senthilkumar P
Dept. of Biotechnology, Hindusthan College of Arts and Science, Coimbatore 641028, TN, India.
Biological Material Laboratory, CSIR-Central Leather Research Institute (CLRI), Chennai 600020, TN, India.
J Photochem Photobiol B. 2018 Feb;179:119-125. doi: 10.1016/j.jphotobiol.2018.01.007. Epub 2018 Jan 16.
In the present day, nanotechnology is one of the most promising leading scientific and potentials areas in modern key technology development toward to the humankind. The synthesis of noble metal nanoparticles (NPs) is an expanding research area due to the possible applications for the development of bio-medical applications. Eco-friendly approach for the biosynthesis of gold nanoparticles (AuNPs) using the aqueous extract from Ruellia tuberosa and Phyllanthus acidus (leaf and twig) for the first time. Surface active AuNPs were characterized by UV-Vis spectroscopy, FTIR (Fourier transform infrared) spectroscopy, DSC (differential scanning colorimetry), DLS (dynamic light scattering) and environmental SEM (scanning electron microscope) analysis at room temperature (RT). Enhanced surface plasmon resonance (SPR) absorbance UV visible optical spectra were detected in the range of 552, 548, 558 and 536 nm. SEM and DLS (transmission mode) analysis confirmed the morphology of the nanoparticles to be spherical with the average size in the range of 88.37, 94.31, 82.23 and 81.36 nm. Further they have enhanced the enzyme activity on α-amylase, cellulase, and xylanase. The results suggest that the phyto-fabricated AuNPs from R. tuberosa and P. acidus is simple, less expensive, eco-friendly, green synthesis and also can be exploited for the potential future industrial and bio-medical applications.
如今，纳米技术是现代关键技术发展中最具前景的前沿科学和潜在领域之一，对人类有着重要意义。由于贵金属纳米颗粒（NPs）在生物医学应用开发方面的潜在应用，其合成是一个不断扩展的研究领域。首次采用来自蓝花草和余甘子（叶和嫩枝）的水提取物，以生态友好的方法生物合成金纳米颗粒（AuNPs）。在室温（RT）下，通过紫外可见光谱、傅里叶变换红外光谱（FTIR）、差示扫描量热法（DSC）、动态光散射（DLS）和环境扫描电子显微镜（SEM）分析对表面活性AuNPs进行了表征。在552、548、558和536nm范围内检测到增强的表面等离子体共振（SPR）吸光度紫外可见光谱。SEM和DLS（透射模式）分析证实纳米颗粒的形态为球形，平均尺寸在88.37、94.31、82.23和81.36nm范围内。此外，它们还增强了α-淀粉酶、纤维素酶和木聚糖酶的酶活性。结果表明，从蓝花草和余甘子中植物合成的AuNPs方法简单、成本低、生态友好、绿色合成，并且可用于未来潜在的工业和生物医学应用。
