LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
J Pharm Biomed Anal. 2018 Mar 20;151:227-234. doi: 10.1016/j.jpba.2018.01.013. Epub 2018 Jan 9.
Collision induced dissociation of triple quadrupole mass spectrometer (CID-QqQ) and high-energy collision dissociation (HCD) of Orbitrap were compared for four neuropeptides Y Y1 (NPY Y1) receptor antagonists and showed similar qualitative fragmentation and structural information. Orbitrap high resolution and high mass accuracy HCD fragmentation spectra allowed unambiguous identification of product ions in the range 0.04-4.25 ppm. Orbitrap mass spectrometry showed abundant analyte-specific product ions also observed on CID-QqQ. These results show the suitability of these product ions for use in quantitative analysis by MRM mode. In addition, it was found that all compounds could be determined at levels >1 μg L using the QqQ instrument and that the detection limits for this analyzer ranged from 0.02 to 0.6 μg L. Overall, the results obtained from experiments acquired in QqQ show a good agreement with those acquired from the Orbitrap instrument allowing the use of this relatively inexpensive technique (QqQ) for accurate quantification of these compounds in clinical and academic applications.
三重四极杆质谱仪（CID-QqQ）的碰撞诱导解离和轨道阱的高能碰撞解离（HCD）对四种神经肽 Y Y1（NPY Y1）受体拮抗剂进行了比较，结果表明它们具有相似的定性裂解和结构信息。轨道阱高分辨率和高质量精度的 HCD 裂解谱图允许在 0.04-4.25ppm 的范围内对产物离子进行明确的鉴定。轨道阱质谱法显示了丰富的分析物特异性产物离子，这些产物离子也在 CID-QqQ 上观察到。这些结果表明这些产物离子适用于通过 MRM 模式进行定量分析。此外，还发现所有化合物都可以在 QqQ 仪器上以 >1μg/L 的水平进行测定，该分析仪的检测限范围为 0.02 至 0.6μg/L。总体而言，在 QqQ 仪器上获得的实验结果与在轨道阱仪器上获得的结果非常吻合，这使得这种相对廉价的技术（QqQ）可用于临床和学术应用中这些化合物的准确定量。
