Lu Zhenxiang, Guo Weina, Liu Chang
College of Animal Science, Anhui Sciences and Technology University, Fengyang, Anhui 233100, China.
J Vet Med Sci. 2018 Mar 24;80(3):427-433. doi: 10.1292/jvms.16-0572. Epub 2018 Jan 23.
In this study, we have identified a bacterium that can inhibit the growth of Staphylococcus aureus, and further analyzed its antibacterial activity and other biological characteristics and laid the foundation for its future application. Through isolation and culture of the unknown bacteria, the culture characteristics, morphology observation, biochemical test, preliminary antibacterial test, 16S rRNA PCR amplification, sequence analysis, and homology analysis were performed. It was found that the bacteria are Gram positive spore chain Bacillus. The bacteria could only ferment glucose for acid production, but could not utilize lactose and maltose. The VP test for this bacteria was positive, while indole and methyl red tests were negative. Further analysis showed that these bacteria shared a homology up to 99.4% with Bacillus subtilis DQ198162.1. Thus, this newly identified bacterium was classified as Bacillus subtilis. Importantly, the crude bacteriocin of this Bacillus subtilis could inhibit the growth of Staphylococcus aureus, Escherichia coli, Enterococcus and Salmonella, which implies its potential usage in the future.
在本研究中，我们鉴定出一种能够抑制金黄色葡萄球菌生长的细菌，并进一步分析了其抗菌活性及其他生物学特性，为其未来应用奠定了基础。通过对未知细菌进行分离培养，开展了培养特性、形态观察、生化试验、初步抗菌试验、16S rRNA PCR扩增、序列分析及同源性分析。结果发现该细菌为革兰氏阳性芽孢链杆菌。该细菌仅能发酵葡萄糖产酸，不能利用乳糖和麦芽糖。此细菌的VP试验呈阳性，而吲哚试验和甲基红试验呈阴性。进一步分析表明，这些细菌与枯草芽孢杆菌DQ198162.1的同源性高达99.4%。因此，这种新鉴定出的细菌被归类为枯草芽孢杆菌。重要的是，这种枯草芽孢杆菌的粗制细菌素能够抑制金黄色葡萄球菌、大肠杆菌、肠球菌和沙门氏菌的生长，这暗示了其在未来的潜在用途。
