Triches T C, Zimmermann G S, de Freitas S F T, Ximenes M, Vieira R S, Cordeiro M M R
Department of Dentistry, Federal University of Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brazil.
, Terra Roxa, Brazil.
Eur Arch Paediatr Dent. 2018 Feb;19(1):47-55. doi: 10.1007/s40368-018-0325-0. Epub 2018 Jan 24.
To determine the efficacy of a single session protocol (SSP) in the reduction of septic content of primary teeth root canals and identify the persistence of bacterial species associated with unsuccessful treatment.
Primary teeth root canals (16) with pulp necrosis and peri-radicular lesions were treated. Samples were collected at baseline (T1), and after chemo-mechanical preparation, before filling (T2). Identification of the microorganisms was determined using checkerboard DNA-DNA hybridisation.
Wilcoxon test was applied for comparison of mean number of species, proportion and mean count of each species between the evaluation times.
Significant reductions were found in the mean number of bacteria species between T1 and T2 (p < 0.05), but not for the reduction in proportion (p > 0.05). There was a reduction (6.0-4.6) of the mean number of species associated with failure, without statistical significance.
The SSP was capable of significantly reducing the septic content, even though, many of the bacteria associated with failure persisted at the time of root canal filling.
确定单次治疗方案（SSP）在降低乳牙根管感染物质含量方面的疗效，并确定与治疗失败相关的细菌种类的持续性。
对16颗患有牙髓坏死和根尖周病变的乳牙根管进行治疗。在基线时（T1）以及化学机械预备后、充填前（T2）采集样本。使用棋盘式DNA-DNA杂交法鉴定微生物。
采用Wilcoxon检验比较各评估时间点细菌种类的平均数、每种细菌的比例和平均计数。
T1和T2之间细菌种类的平均数显著减少（p < 0.05），但比例降低无统计学意义（p > 0.05）。与失败相关的细菌种类平均数有所减少（6.0 - 4.6），但无统计学意义。
单次治疗方案能够显著降低感染物质含量，尽管在根管充填时，许多与治疗失败相关的细菌仍然存在。
