de Sousa Denise Lins, de Sousa Rebecca Bastos Rocha Araújo, Pinto Daniela Nunes, Neto José Jeová Siebra Moreira, de Carvalho Cibele Barreto Mano, de Almeida Paulo César
Department of Dental Clinics, School of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Pediatr Dent. 2011 Jul-Aug;33(4):307-11.
This study's purposes were to: evaluate the antibacterial effect of chemomechanical instrumentation and a calcium hydroxide-based dressing in primary teeth with pulp necrosis secondary to trauma; and detect the presence of Fusobacterium nucleatum and black-pigmented rods in the canals of these teeth.
Microbiological sample collections (MSCs) were obtained: after coronal access to the canals of primary incisors (in 18 teeth; MSC 1); after chemomechanical instrumentation (in 10 teeth; MSC 2); and 72 hours after removal of intracanal medication (in 18 teeth; MSC 3). These samples were sent for microbiological processing. The results were analyzed statistically via chi-square, analysis of variance, and Games-Howell tests (P<.05).
Micro-organisms were isolated in approximately 94%, 10%, and 83% of canals, respectively, in MSCs 1, 2, and 3. There was a statistically significant difference only between MSCs 1 and 2 (P<.03). F nucleatum and black-pigmented rods were detected in approximately 56% and 11% of canals in MSC 1, respectively.
Chemomechanical instrumentation and calcium hydroxide-based dressing have an anti-bacterial effect by significantly reducing the number of micro-organisms in the main root canal. They showed a limited efficacy, however, and did not prevent bacterial regrowth after endodontic therapy in primary teeth with pulp necrosis secondary to trauma.
本研究的目的是：评估化学机械预备和氢氧化钙类根管封药对因外伤导致牙髓坏死的乳磨牙的抗菌效果；并检测这些牙齿根管中具核梭杆菌和产黑色素杆菌的存在情况。
获取微生物样本：在对乳前牙根管进行开髓后（18颗牙齿；样本1）；在进行化学机械预备后（10颗牙齿；样本2）；以及在去除根管内药物72小时后（18颗牙齿；样本3）。将这些样本送去进行微生物学处理。通过卡方检验、方差分析和Games-Howell检验对结果进行统计学分析（P<0.05）。
在样本1、2和3中，分别约有94%、10%和83%的根管中分离出微生物。仅样本1和样本2之间存在统计学显著差异（P<0.03）。在样本1中，分别约有56%和11%的根管中检测到具核梭杆菌和产黑色素杆菌。
化学机械预备和氢氧化钙类根管封药通过显著减少主根管内微生物数量而具有抗菌效果。然而，它们的疗效有限，并且不能防止外伤继发牙髓坏死的乳磨牙根管治疗后细菌的再生长。
