Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7, 87-100, Toruń, Poland.
Interdisciplinary Centre for Modern Technologies, Nicolaus Copernicus University, Wileńska 4, 87-100, Toruń, Poland.
Anal Bioanal Chem. 2018 Feb;410(5):1571-1582. doi: 10.1007/s00216-017-0807-7. Epub 2018 Jan 24.
Endometrial cancer is one of the most commonly diagnosed cancers in women. The search for factors that contribute to the development of cancer cells in reproductive organs should involve the detection of xenoestrogens, in particular zearalenone (ZEA) and its metabolites. Xenoestrogens are endocrine disruptors-ZEA and its metabolites are structurally similar to estrogens (macrocyclic lactone ring) and show high affinity for estrogen receptors. This study proposes a new method for the preparation of samples of human tissues with endometrial cancer by the use of the QuEChERS technique. Analytical parameters such as centrifugation temperature, extraction solvent, and adsorbents were modified to obtain satisfactory recovery for ZEA (R = 82.6%, RSD = 2.9%) and one of its metabolites, α-zearalenol (R = 50.1%, RSD = 3.2%). High-performance liquid chromatography (HPLC) with fluorescence detection and tandem mass spectrometry (LC-QTOF-MS) were used for the identification and quantitative determination of the analyzed compounds. The developed procedure was applied for analyses of human tissues with endometrial cancer. The presence of α-zearalenol was detected in 47 out of the 61 examined tissue samples. Graphical Abstract Methodology for isolation and identification of zearalenone and its major metabolites.
子宫内膜癌是女性最常见的癌症之一。寻找导致生殖器官癌细胞发展的因素，应包括检测外源性雌激素，特别是玉米赤霉烯酮（ZEA）及其代谢物。外源性雌激素是内分泌干扰物——ZEA 及其代谢物的结构与雌激素（大环内酯环）相似，对雌激素受体具有高亲和力。本研究提出了一种新的方法，通过 QuEChERS 技术制备患有子宫内膜癌的人体组织样本。对离心温度、提取溶剂和吸附剂等分析参数进行了改进，以获得 ZEA（R = 82.6%，RSD = 2.9%）和其一种代谢物α-玉米赤霉烯醇（R = 50.1%，RSD = 3.2%）的满意回收率。采用高效液相色谱（HPLC）-荧光检测和串联质谱（LC-QTOF-MS）对分析物进行鉴定和定量测定。所开发的方法用于分析患有子宫内膜癌的人体组织。在 61 个检测的组织样本中，有 47 个检测到了α-玉米赤霉烯醇。
