一种同时检测正常和肿瘤性小鼠造血细胞上Fc受体和分化抗原的简单方法。
A simple method for simultaneous detection of Fc receptors and differentiation antigens on normal and neoplastic murine hematopoietic cells.
作者信息
Siden E, Siegel M
出版信息
J Immunol Methods. 1986 Mar 13;87(2):251-5. doi: 10.1016/0022-1759(86)90539-9.
PMID:2936827
Abstract
Cell surface receptors for immunoglobulin Fc regions can be detected by light microscopy of cytocentrifuge preparations of normal or malignant murine hematopoietic cells. The cells are first incubated in a culture supernate containing a monoclonal anti-hapten antibody, then with heat-killed, haptenated bacteria. Using 2 morphologically distinct strains of bacteria, we can detect both Fc receptors and cell surface differentiation antigens.
摘要
免疫球蛋白Fc区域的细胞表面受体可通过对正常或恶性小鼠造血细胞的细胞离心涂片进行光学显微镜检查来检测。首先将细胞在含有单克隆抗半抗原抗体的培养上清液中孵育，然后与经热灭活的、结合了半抗原的细菌一起孵育。使用两种形态不同的细菌菌株，我们能够检测Fc受体和细胞表面分化抗原。
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