Kornfeld S, Beug H, Doederlein G, Graf T
Exp Cell Res. 1983 Feb;143(2):383-94. doi: 10.1016/0014-4827(83)90065-4.
The isolation and characterization of monoclonal antibodies reacting with cell surface antigenic determinants of normal and leukemic avian hematopoietic cells is described. The antibodies were produced by immunizing mice with normal macrophages, as well as with myeloid cells transformed with the avian acute leukemia viruses MC29, AMV and E26. Eleven antibodies were characterized for their reactivity with a variety of normal and leukemic cells of the myeloid, B- and T-lymphoid and of the erythroid cell lineage. Using several methods, they could be subdivided into five distinct types: I. Four antibodies were specific for the myeloid lineage, predominantly reacting with immature myeloid cells. II. One antibody reacted with mature and immature myeloid cells as well as with T-lymphoid cells. III. Four antibodies reacted with myeloid, erythroid and T-lymphoid cells. IV. One antibody reacted with myeloid as well as with T- and B-lymphoid cells. V. One antibody reacted with all kinds of chicken hematopoietic cells except erythrocytes. The first type of antibodies detected glycoproteins with MWs of 170 and 130 kD. The pattern of antigens precipitated varied with the different monoclonal antibodies of this group. The antibody of the fourth type precipitated a 30 kD polypeptide from extracts of myeloid and lymphoid cells. None of the other antibodies precipitated any detectable proteins.
本文描述了与正常及白血病禽类造血细胞的细胞表面抗原决定簇发生反应的单克隆抗体的分离和特性鉴定。这些抗体是通过用正常巨噬细胞以及用禽急性白血病病毒MC29、AMV和E26转化的髓样细胞免疫小鼠而产生的。对11种抗体与髓样、B淋巴细胞和T淋巴细胞以及红系细胞系的多种正常和白血病细胞的反应性进行了特性鉴定。通过几种方法，它们可被分为五种不同类型：I. 四种抗体对髓样细胞系具有特异性，主要与未成熟髓样细胞发生反应。II. 一种抗体与成熟和未成熟髓样细胞以及T淋巴细胞发生反应。III. 四种抗体与髓样、红系和T淋巴细胞发生反应。IV. 一种抗体与髓样以及T和B淋巴细胞发生反应。V. 一种抗体与除红细胞外的所有鸡造血细胞发生反应。第一类抗体检测到分子量为170和130 kD的糖蛋白。该组不同单克隆抗体沉淀的抗原模式各不相同。第四类抗体从髓样和淋巴细胞提取物中沉淀出一种30 kD的多肽。其他抗体均未沉淀出任何可检测到的蛋白质。
