Department of Endodontics, Faculty of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Clinic for Nutrition and Natural Medicine (COM Clinic), Karaj, Iran.
Phytother Res. 2018 Apr;32(4):672-677. doi: 10.1002/ptr.6015. Epub 2018 Jan 24.
The aim of this study was to determine the antimicrobial capacity, minimum inhibitory concentration (MIC), and cytotoxic effects of a Peganum harmala seed extract in comparison to 5.25% sodium hypochlorite (NaOCl). The oral pathogen Enterococcus faecalis was used to evaluate the antimicrobial capacity, and the MIC values were determined through serial dilution. Inhibition zones were measured in millimeter, and the data were analyzed statistically by analysis of variance and the Tukey HSD test. For cytotoxicity testing, P. harmala seed extract and 5.25% NaOCl solution were incubated with L929 fibroblast cells. After 1, 24, and 72 hr of incubation, cells were stained and the optical density determined with an enzyme-linked immunosorbent assay (ELISA) reader. Data were analyzed with Chi-Square statistical test. The significance level was set at p < .05. There was no significant difference between the antimicrobial capacity of 5.25% NaOCl and the P. harmala extract (p > .05; MIC 4 μg/ml). The Microculture Tetrazolium (MTT) assay test showed that the cytotoxic effects of the P. harmala extract were significantly lower than 5.25% NaOCl (p < .05). The results show that 5.25% NaOCl and P. harmala seed extract have similar antimicrobial activity against Enterococcus faecalis; but P. harmala, which shows reduced cytotoxicity, should be considered for further investigation as a safe, phytotherapeutic, intracanal irrigant.
本研究旨在比较贯叶连翘种子提取物与 5.25%次氯酸钠（NaOCl）的抗菌能力、最小抑菌浓度（MIC）和细胞毒性作用。采用口腔病原体粪肠球菌评估抗菌能力，通过连续稀释法测定 MIC 值。以毫米为单位测量抑菌圈，数据采用方差分析和 Tukey HSD 检验进行统计学分析。为了进行细胞毒性测试，将贯叶连翘种子提取物和 5.25% NaOCl 溶液与 L929 成纤维细胞孵育。孵育 1、24 和 72 小时后，用酶联免疫吸附试验（ELISA）读取器对细胞进行染色并测定吸光度。采用卡方检验进行数据分析。显著水平设为 p<.05。5.25% NaOCl 和贯叶连翘提取物的抗菌能力无显著差异（p>.05；MIC 为 4μg/ml）。微量细胞培养四唑盐（MTT）检测显示，贯叶连翘提取物的细胞毒性作用明显低于 5.25% NaOCl（p<.05）。结果表明，5.25% NaOCl 和贯叶连翘种子提取物对粪肠球菌具有相似的抗菌活性；但贯叶连翘的细胞毒性较低，应作为一种安全、植物疗法、根管冲洗剂进一步研究。
