特异性位点测试表明，乙基亚硝基脲是小鼠中最有效的诱变剂。
Specific-locus test shows ethylnitrosourea to be the most potent mutagen in the mouse.
作者信息
Russell W L, Kelly E M, Hunsicker P R, Bangham J W, Maddux S C, Phipps E L
出版信息
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5818-9. doi: 10.1073/pnas.76.11.5818.
Abstract
Use of the specific-locus test to measure the frequency of transmitted gene mutations induced in mouse spermatogonia has shown ethylnitrosourea to be by far the most potent mutagen yet discovered in the mouse. The dose used, 250 mg/kg, gave a mutation rate 5 times as high as had been obtained with 600 R, the most effective acute dose of x-rays. Compared to procarbazine, heretofore the most mutagenic chemical known in the mouse, ethylnitrosourea proved to be 15 times more mutagenic than the peak effect obtained with the most effective dose of procarbazine. Because of its high mutagenicity, ethylnitrosourea can serve as a model compound in exploring the effect of such factors as dose response, dose fractionation, sex, and cell stage on the mutagenic action of a chemical. Ethylnitrosourea is clearly the mutagen of choice for the production of any kind of desired new gene mutations in the mouse.
摘要
运用特定位点测试来测定在小鼠精原细胞中诱发的可传递基因突变的频率，结果表明，迄今为止，在小鼠中发现的最有效的诱变剂是乙基亚硝基脲。所使用的剂量为250毫克/千克，其诱发突变的速率比600伦琴（X射线最有效的急性剂量）所产生的速率高5倍。与丙卡巴肼相比（丙卡巴肼此前是小鼠中已知的最具诱变作用的化学物质），乙基亚硝基脲的诱变作用比丙卡巴肼最有效剂量所产生的峰值效应还要高15倍。由于其高诱变活性，乙基亚硝基脲可作为一种模型化合物，用于探究诸如剂量反应、剂量分割、性别和细胞阶段等因素对化学物质诱变作用的影响。显然，乙基亚硝基脲是在小鼠中产生任何所需新基因突变的首选诱变剂。
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