乙基亚硝基脲诱导小鼠精原细胞特异性位点突变的剂量-反应曲线。
Dose--response curve for ethylnitrosourea-induced specific-locus mutations in mouse spermatogonia.
作者信息
Russell W L, Hunsicker P R, Raymer G D, Steele M H, Stelzner K F, Thompson H M
出版信息
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3589-91. doi: 10.1073/pnas.79.11.3589.
Abstract
The extreme mutagenic effectiveness of N-ethyl-N-nitrosourea in the mouse has permitted the accumulation of the most extensive dose--response data yet obtained for chemical induction of specific-locus mutations in spermatogonia. In the lower portion of the curve, below a dose of 100 mg/kg, the data fall statistically significantly below a maximum likelihood fit to a straight line. Independent evidence indicates that, over this dose range, ethylnitrosourea reaches the testis in amounts directly proportional to the injected dose. It is concluded that, despite the mutagenic effectiveness of ethylnitrosourea, the spermatogonia are apparently capable of repairing at least a major part of the mutational damage when the repair process is not swamped by a high dose. This finding is important both in basic studied on the mutagenic action of chemicals in mammals and in risk estimation.
摘要
N-乙基-N-亚硝基脲对小鼠具有极高的诱变效力，这使得人们积累了迄今为止关于化学诱导精原细胞特异性位点突变最为广泛的剂量-反应数据。在曲线较低部分，低于100mg/kg的剂量时，数据在统计学上显著低于对直线的最大似然拟合。独立证据表明，在这个剂量范围内，乙基亚硝基脲进入睾丸的量与注射剂量成正比。可以得出结论，尽管乙基亚硝基脲具有诱变效力，但当修复过程未被高剂量淹没时，精原细胞显然能够修复至少大部分的突变损伤。这一发现对于哺乳动物化学诱变作用的基础研究以及风险评估都很重要。
相似文献
1
Dose--response curve for ethylnitrosourea-induced specific-locus mutations in mouse spermatogonia.乙基亚硝基脲诱导小鼠精原细胞特异性位点突变的剂量-反应曲线。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3589-91. doi: 10.1073/pnas.79.11.3589.
2
Effect of dose fractionation on the ethylnitrosourea induction of specific-locus mutations in mouse spermatogonia.剂量分割对乙基亚硝基脲诱导小鼠精原细胞特定位点突变的影响。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3592-3. doi: 10.1073/pnas.79.11.3592.
3
Specific-locus test shows ethylnitrosourea to be the most potent mutagen in the mouse.特异性位点测试表明，乙基亚硝基脲是小鼠中最有效的诱变剂。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5818-9. doi: 10.1073/pnas.76.11.5818.
4
Dose-repetition increases the mutagenic effectiveness of N-ethyl-N-nitrosourea in mouse spermatogonia.剂量重复增加了N-乙基-N-亚硝基脲对小鼠精原细胞的诱变效力。
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6619-21. doi: 10.1073/pnas.82.19.6619.
5
A dose-response analysis of ethylnitrosourea-induced recessive specific-locus mutations in treated spermatogonia of the mouse.
Mutat Res. 1990 Jul;231(1):47-54. doi: 10.1016/0027-5107(90)90175-4.
6
The use of two-dimensional electrophoresis to detect mutations induced in mouse spermatogonia by ethylnitrosourea.利用二维电泳检测乙基亚硝基脲诱导小鼠精原细胞产生的突变。
Can J Genet Cytol. 1983 Oct;25(5):457-66. doi: 10.1139/g83-070.
7
Electrophoretically detected germinal mutations induced in the mouse by ethylnitrosourea.电泳检测到的乙基亚硝基脲在小鼠中诱导产生的生殖细胞突变。
Proc Natl Acad Sci U S A. 1981 May;78(5):3138-41. doi: 10.1073/pnas.78.5.3138.
8
Comparative studies of dose-response curves for recessive lethal mutations induced by ethylnitrosourea in spermatogonia and in spermatozoa of Drosophila melanogaster.对黑腹果蝇精原细胞和精子中乙基亚硝基脲诱导的隐性致死突变剂量反应曲线的比较研究。
Environ Mutagen. 1984;6(4):489-96. doi: 10.1002/em.2860060403.
9
The frequency of dominant cataract and recessive specific-locus mutations in mice derived from 80 or 160 mg ethylnitrosourea per kg body weight treated spermatogonia.源自每千克体重经80或160毫克乙基亚硝基脲处理的精原细胞的小鼠中显性白内障和隐性特定位点突变的频率。
Mutat Res. 1986 Aug;162(1):69-80. doi: 10.1016/0027-5107(86)90072-2.
10
Supermutagenicity of ethylnitrosourea in the mouse spot test: comparisons with methylnitrosourea and ethylnitrosourethane.小鼠斑点试验中乙基亚硝基脲的超强诱变活性：与甲基亚硝基脲和乙基亚硝基脲烷的比较。
Mutat Res. 1982 Feb 22;92(1-2):193-204. doi: 10.1016/0027-5107(82)90223-8.
引用本文的文献
1
Studies on the Small Body Size Mouse Developed by Mutagen -Ethyl--nitrosourea.诱变剂-乙基-亚硝基脲诱导产生的小体型小鼠的研究
Toxicol Res. 2008 Mar;24(1):69-78. doi: 10.5487/TR.2008.24.1.069. Epub 2008 Mar 1.
2
Mouse Genome Informatics (MGI) Resource: Genetic, Genomic, and Biological Knowledgebase for the Laboratory Mouse.小鼠基因组信息学（MGI）资源：实验室小鼠的遗传、基因组和生物学知识库。
ILAR J. 2017 Jul 1;58(1):17-41. doi: 10.1093/ilar/ilx013.
3
Genomic characterization of mutant laboratory mouse strains by exome sequencing and annotation lift-over.通过外显子组测序和注释转换对突变实验室小鼠品系进行基因组特征分析。
BMC Genomics. 2015 May 6;16(1):351. doi: 10.1186/s12864-015-1548-7.
4
Sublinear response in lacZ mutant frequency of Muta™ Mouse spermatogonial stem cells after low dose subchronic exposure to N-ethyl-N-nitrosourea.低剂量亚慢性暴露于N-乙基-N-亚硝基脲后，Muta™小鼠精原干细胞lacZ突变频率的亚线性反应。
Environ Mol Mutagen. 2015 May;56(4):347-55. doi: 10.1002/em.21932. Epub 2015 Jan 17.
5
Transgenic rodent assay for quantifying male germ cell mutant frequency.用于定量雄性生殖细胞突变频率的转基因啮齿动物试验。
J Vis Exp. 2014 Aug 6(90):e51576. doi: 10.3791/51576.
6
Mouse mutagenesis with the chemical supermutagen ENU.利用化学超级诱变剂ENU进行小鼠诱变。
Methods Enzymol. 2010;477:297-312. doi: 10.1016/S0076-6879(10)77015-4.
7
A mouse model for Meckel syndrome reveals Mks1 is required for ciliogenesis and Hedgehog signaling.梅克尔综合征的小鼠模型表明 Mks1 对于纤毛发生和 Hedgehog 信号通路是必需的。
Hum Mol Genet. 2009 Dec 1;18(23):4565-75. doi: 10.1093/hmg/ddp422. Epub 2009 Sep 22.
8
Comparison of the genetic effects of equimolar doses of ENU and MNU: while the chemicals differ dramatically in their mutagenicity in stem-cell spermatogonia, both elicit very high mutation rates in differentiating spermatogonia.等摩尔剂量的ENU和MNU遗传效应比较：尽管这两种化学物质在干细胞精原细胞中的致突变性差异显著，但它们在分化中的精原细胞中均引发非常高的突变率。
Mutat Res. 2007 Mar 1;616(1-2):181-95. doi: 10.1016/j.mrfmmm.2006.11.003. Epub 2006 Dec 14.
9
An allelic series of mutations in the Kit ligand gene of mice. II. Effects of ethylnitrosourea-induced Kitl point mutations on survival and peripheral blood cells of Kitl(Steel) mice.小鼠Kit配体基因中的一系列等位基因突变。II. 乙基亚硝基脲诱导的Kitl点突变对Kitl(Steel)小鼠存活及外周血细胞的影响。
Genetics. 2002 Sep;162(1):341-53. doi: 10.1093/genetics/162.1.341.
10
An allelic series of mutations in the kit ligand gene of mice. I. Identification of point mutations in seven ethylnitrosourea-induced Kitl(Steel) alleles.小鼠kit配体基因中的一系列等位基因突变。I. 七个乙基亚硝基脲诱导的Kitl(Steel)等位基因中的点突变鉴定。
Genetics. 2002 Sep;162(1):331-40. doi: 10.1093/genetics/162.1.331.
本文引用的文献
1
X-ray-induced mutations in mice.X射线诱导的小鼠突变。
Cold Spring Harb Symp Quant Biol. 1951;16:327-36. doi: 10.1101/sqb.1951.016.01.024.
2
Mutation-rate determinations based on electrophoretic analysis of laboratory mice.
Mutat Res. 1981 Jun;82(1):125-135. doi: 10.1016/0027-5107(81)90144-5.
3
Effect of dose fractionation on the ethylnitrosourea induction of specific-locus mutations in mouse spermatogonia.剂量分割对乙基亚硝基脲诱导小鼠精原细胞特定位点突变的影响。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3592-3. doi: 10.1073/pnas.79.11.3592.
4
Procarbazine-induced specific-locus mutations in male mice.甲基苄肼诱导雄性小鼠的特定基因座突变。
Mutat Res. 1979 Feb;59(2):245-56. doi: 10.1016/0027-5107(79)90163-5.
5
Specific-locus test shows ethylnitrosourea to be the most potent mutagen in the mouse.特异性位点测试表明，乙基亚硝基脲是小鼠中最有效的诱变剂。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5818-9. doi: 10.1073/pnas.76.11.5818.
Zotero 插件