Buckingham J C
Neuroendocrinology. 1986;42(2):148-52. doi: 10.1159/000124266.
The influence of beta-endorphin on the secretion of corticotrophin releasing factor (CRF) by isolated rat hypothalami in vitro was studied. beta-Endorphin (10(-11)-10(-10) M) caused dose-related increases in the CRF contents of the hypothalami and of the medium in which they were incubated. Its effects were antagonized by naloxone (10(-8)-10(-7) M). In contrast, in higher concentrations (10(-7) - 10(-5) M), it reduced, in a dose-dependent manner, both the spontaneous release of CRF from the hypothalami and the release which normally occurred in response to acetylcholine, 5-hydroxytryptamine, morphine, met-enkephalin and leu-enkephalin. The inhibition of CRF release was associated with a rise in the tissue content of the hormone and was not blocked readily by naloxone. The results support the concept that opioid substances may be involved in the control of hypothalamo-pituitary-adrenocortical function.
研究了β-内啡肽对体外培养的大鼠离体下丘脑促肾上腺皮质激素释放因子（CRF）分泌的影响。β-内啡肽（10⁻¹¹ - 10⁻¹⁰ M）使下丘脑及其孵育培养基中的CRF含量呈剂量依赖性增加。其作用被纳洛酮（10⁻⁸ - 10⁻⁷ M）拮抗。相反，在较高浓度（10⁻⁷ - 10⁻⁵ M）时，它以剂量依赖的方式降低了下丘脑CRF的自发释放以及通常由乙酰胆碱、5-羟色胺、吗啡、甲硫氨酸脑啡肽和亮氨酸脑啡肽引起的释放。CRF释放的抑制与激素的组织含量增加有关，且不易被纳洛酮阻断。这些结果支持了阿片类物质可能参与下丘脑-垂体-肾上腺皮质功能控制的观点。
