晶体和溶液中羧基肌红蛋白的结构。
Structure of carboxymyoglobin in crystals and in solution.
作者信息
Makinen M W, Houtchens R A, Caughey W S
出版信息
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6042-6. doi: 10.1073/pnas.76.12.6042.
Abstract
The configuration of the heme-carbonyl group upon binding of carbon monoxide to sperm whale myoglobin (Mb) in crystals is evaluated on the basis of infrared spectroscopic methods. Multiplets of the totally symmetric C-O stretching mode are observed for the heme-bound ligand near 1933, 1944, and 1967 cm-1, corresponding to three different heme-carbonyl conformers. Variations in the relative proportions of these conformers can be induced by incorporation of small fractions of metMb or deoxyMb into MbCO crystals. The configuration of the iron-carbonyl with respect to the immediate coordination environment of the heme iron is assigned for each v(CO) stretching frequency on the basis of a detailed comparison of the three-dimensional structures of the heme environments of MbCO, metMb, and deoxyMb defined by crystallographic methods. The structures of the three heme-carbonyl conformers account for the v(CO) infrared absorption bands that can be observed for MbCO in solution.
摘要
基于红外光谱方法，对一氧化碳与晶体中的抹香鲸肌红蛋白（Mb）结合时血红素 - 羰基基团的构型进行了评估。在1933、1944和1967 cm-1附近观察到与血红素结合的配体的全对称C - O伸缩模式的多重峰，对应于三种不同的血红素 - 羰基构象体。通过将少量高铁肌红蛋白（metMb）或脱氧肌红蛋白（deoxyMb）掺入MbCO晶体中，可以诱导这些构象体相对比例的变化。基于通过晶体学方法确定的MbCO、metMb和deoxyMb血红素环境的三维结构的详细比较，为每个v(CO)伸缩频率指定了相对于血红素铁直接配位环境的铁 - 羰基构型。三种血红素 - 羰基构象体的结构解释了在溶液中可观察到的MbCO的v(CO)红外吸收带。
相似文献
1
Structure of carboxymyoglobin in crystals and in solution.晶体和溶液中羧基肌红蛋白的结构。
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6042-6. doi: 10.1073/pnas.76.12.6042.
2
Crystal structures of CO-, deoxy- and met-myoglobins at various pH values.不同pH值下一氧化碳肌红蛋白、脱氧肌红蛋白和高铁肌红蛋白的晶体结构。
J Mol Biol. 1996 Mar 8;256(4):762-74. doi: 10.1006/jmbi.1996.0123.
3
Iron-carbonyl bond geometries of carboxymyoglobin and carboxyhemoglobin in solution determined by picosecond time-resolved infrared spectroscopy.通过皮秒时间分辨红外光谱法测定溶液中羧基肌红蛋白和羧基血红蛋白的铁羰基键几何结构。
Proc Natl Acad Sci U S A. 1988 Jul;85(14):5062-6. doi: 10.1073/pnas.85.14.5062.
4
Spectroscopic studies of myoglobin at low pH: heme structure and ligation.低pH值下肌红蛋白的光谱研究：血红素结构与连接
Biochemistry. 1991 Feb 5;30(5):1227-37. doi: 10.1021/bi00219a010.
5
Carbon monoxide binding properties of domain-swapped dimeric myoglobin.结构域交换二聚体肌红蛋白的一氧化碳结合特性
J Biol Inorg Chem. 2015 Apr;20(3):523-30. doi: 10.1007/s00775-014-1236-0. Epub 2015 Jan 13.
6
Infrared spectroscopy of photodissociated carboxymyoglobin at low temperatures.低温下光解离羧基肌红蛋白的红外光谱
Proc Natl Acad Sci U S A. 1982 Jun;79(12):3744-8. doi: 10.1073/pnas.79.12.3744.
7
Modification of the distal histidyl imidazole in myoglobin to N-tetrazole-substituted imidazole and its effects on the heme environmental structure and ligand binding properties.肌红蛋白中远端组氨酸咪唑修饰为N-四唑取代的咪唑及其对血红素环境结构和配体结合特性的影响。
Biochemistry. 1992 Sep 15;31(36):8613-8. doi: 10.1021/bi00151a031.
8
Ligand binding to synthetic mutant myoglobin (His-E7----Gly): role of the distal histidine.配体与合成突变体肌红蛋白（组氨酸-E7→甘氨酸）的结合：远端组氨酸的作用
Proc Natl Acad Sci U S A. 1988 Nov;85(22):8497-501. doi: 10.1073/pnas.85.22.8497.
9
Crystal structures of myoglobin-ligand complexes at near-atomic resolution.肌红蛋白-配体复合物的近原子分辨率晶体结构。
Biophys J. 1999 Oct;77(4):2153-74. doi: 10.1016/S0006-3495(99)77056-6.
10
Pressure effects on the proximal heme pocket in myoglobin probed by Raman and near-infrared absorption spectroscopy.通过拉曼光谱和近红外吸收光谱探究压力对肌红蛋白近端血红素口袋的影响。
Biophys J. 1997 Nov;73(5):2752-63. doi: 10.1016/S0006-3495(97)78304-8.
引用本文的文献
1
Dynamics of a myoglobin mutant enzyme: 2D IR vibrational echo experiments and simulations.肌红蛋白突变酶的动力学：2D IR 振动回波实验和模拟。
J Am Chem Soc. 2010 Dec 29;132(51):18367-76. doi: 10.1021/ja108491t. Epub 2010 Dec 8.
2
Role of solvent on protein-matrix coupling in MbCO embedded in water-saccharide systems: a Fourier transform infrared spectroscopy study.溶剂对嵌入水-糖体系中的肌红蛋白一氧化碳（MbCO）中蛋白质-基质偶联的作用：傅里叶变换红外光谱研究
Biophys J. 2006 Aug 1;91(3):968-80. doi: 10.1529/biophysj.106.081927. Epub 2006 May 19.
3
Heme carbonyls: environmental effects on nu(C-O) and Fe-C/C-O bond length correlations.血红素羰基化合物：环境对ν（C-O）和Fe-C/C-O键长相关性的影响。
J Am Chem Soc. 2005 Oct 19;127(41):14422-33. doi: 10.1021/ja053148x.
4
Electron transfer kinetics in photosynthetic reaction centers embedded in trehalose glasses: trapping of conformational substates at room temperature.嵌入海藻糖玻璃中的光合反应中心的电子转移动力学：室温下构象亚态的捕获
Biophys J. 2002 Feb;82(2):558-68. doi: 10.1016/S0006-3495(02)75421-0.
5
Molecular dynamics simulation of carboxy-myoglobin embedded in a trehalose-water matrix.嵌入海藻糖 - 水基质中的羧基肌红蛋白的分子动力学模拟
Biophys J. 2001 Feb;80(2):931-8. doi: 10.1016/S0006-3495(01)76072-9.
6
Crystal structures of myoglobin-ligand complexes at near-atomic resolution.肌红蛋白-配体复合物的近原子分辨率晶体结构。
Biophys J. 1999 Oct;77(4):2153-74. doi: 10.1016/S0006-3495(99)77056-6.
7
Connection between the taxonomic substates and protonation of histidines 64 and 97 in carbonmonoxy myoglobin.一氧化碳肌红蛋白中分类亚态与组氨酸64和97质子化之间的联系。
Biophys J. 1999 Aug;77(2):1036-51. doi: 10.1016/s0006-3495(99)76954-7.
8
Dehydration and crystallization of trehalose and sucrose glasses containing carbonmonoxy-myoglobin.含有一氧化碳肌红蛋白的海藻糖和蔗糖玻璃态物质的脱水与结晶
Biophys J. 1999 May;76(5):2727-34. doi: 10.1016/S0006-3495(99)77425-4.
9
Ligand binding to heme proteins. VI. Interconversion of taxonomic substates in carbonmonoxymyoglobin.配体与血红素蛋白的结合。VI. 一氧化碳肌红蛋白中分类亚态的相互转化。
Biophys J. 1996 Sep;71(3):1563-73. doi: 10.1016/S0006-3495(96)79359-1.
10
Theoretical study of the distal-side steric and electrostatic effects on the vibrational characteristics of the FeCO unit of the carbonylheme proteins and their models.远端空间位阻和静电作用对羰基血红素蛋白及其模型中FeCO单元振动特性影响的理论研究
Biophys J. 1996 Mar;70(3):1214-29. doi: 10.1016/S0006-3495(96)79680-7.
本文引用的文献
1
Analysis of the principal g-tensors in single crystals of ferrimyoglobin complexes.铁肌红蛋白配合物单晶中主要g张量的分析。
Biochim Biophys Acta. 1971 Nov 19;251(2):227-35. doi: 10.1016/0005-2795(71)90106-1.
2
An infrared study of bound carbon monoxide in the human red blood cell, isolated hemoglobin, and heme carbonyls.对人体红细胞、分离的血红蛋白和血红素羰基化合物中结合一氧化碳的红外研究。
Biochemistry. 1968 Jan;7(1):175-83. doi: 10.1021/bi00841a022.
3
Structures of deoxy- and carbonmonoxy-erythrocruorin.脱氧和一氧化碳合红血球铁蛋白的结构
J Mol Biol. 1970 Sep 14;52(2):349-54. doi: 10.1016/0022-2836(70)90035-5.
4
Optically detected conformational changes in haemoglobin single crystals.血红蛋白单晶中光学检测到的构象变化。
Nature. 1974 Jan 4;247(5435):62-4. doi: 10.1038/247062a0.
5
An infrared study of NO bonding to heme B and hemoglobin A. Evidence for inositol hexaphosphate induced cleavage of proximal histidine to iron bonds.一氧化氮与血红素B和血红蛋白A结合的红外光谱研究。肌醇六磷酸诱导近端组氨酸与铁键断裂的证据。
Biochemistry. 1976 Jan 27;15(2):388-96. doi: 10.1021/bi00647a023.
6
Neutron diffraction analysis of myoglobin: structure of the carbon monoxide derivative.肌红蛋白的中子衍射分析：一氧化碳衍生物的结构
Science. 1975 Nov 7;190(4214):568-70. doi: 10.1126/science.1188354.
7
Stereochemistry of carbonylmetalloporphyrins. The structure of (pyridine)(carbonyl)(5, 10, 15, 20-tetraphenylprophinato)iron(II).羰基金属卟啉的立体化学。（吡啶）（羰基）（5,10,15,20-四苯基卟吩inato）铁（II）的结构。
J Am Chem Soc. 1976 Dec 8;98(25):8032-6. doi: 10.1021/ja00441a025.
8
Structure of horse carbonmonoxyhaemoglobin.马碳氧血红蛋白的结构
J Mol Biol. 1976 Jul 5;104(3):707-22. doi: 10.1016/0022-2836(76)90130-3.
9
Structure of cyanide methemoglobin.氰化高铁血红蛋白的结构
J Mol Biol. 1976 Jul 5;104(3):687-706. doi: 10.1016/0022-2836(76)90129-7.
10
Structure of myoglobin refined at 2-0 A resolution. II. Structure of deoxymyoglobin from sperm whale.肌红蛋白结构在2.0埃分辨率下的精修。II. 抹香鲸脱氧肌红蛋白的结构。
J Mol Biol. 1977 Mar 5;110(3):569-84. doi: 10.1016/s0022-2836(77)80112-5.
Zotero 插件